Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No.977 Park Road, Yanji, 133000, China.
Jilin Provincial Academic of Animal Husbandry and Veterinary Medicine, Changchun, 130062, China.
Sci Rep. 2022 Jul 30;12(1):13121. doi: 10.1038/s41598-022-16658-6.
Toxoplasma gondii, one of the important zoonotic parasites, has been detected in lots of hosts including humans, with a widespread prevalence. The products of equids, such as meat and milk, have been closely related to humans' life. As the intermediate hosts, little is known about equids toxoplasmosis in Jilin province. Therefore, the present study aimed to evaluate the occurrence and risk factors of Toxoplasma gondii infections in equids from Jilin, northeastern China. In this study, a total of 245 blood samples of equids (192 horses, 25 donkeys and 28 mules) were collected from six localities in Jilin Province from March 2018 to August 2020 and detected by PCR. The occurrence rate of T. gondii B1 gene was analyzed using multivariable logistic regression to evaluate risk factors associated with the positive rates in equids. Among 245equids, T. gondii molecular occurrence was 9.0% (22/245). The highest positive rate was observed in equids from Dongfeng (16.3%) followed by Taonan (10.0%), Wangqing (8.3%), Antu (8.0%), Tonghua (8.0%) and Shulan (2.3%). Statistical analysis revealed that farming model and region may be two main risk factors. Data analysis indicated that the positive rate in captive farm (3.2%, 95% CI: 0.0-6.7%) was significantly lower than those in cage-free farm (P < 0.05), and the region of Shulan was protective factor (OR: 0.063, 95% CI: 0.007-0.559).The results of our study alert people to be aware that the present of equids T. gondii infection in this region, and contribute to a prevention and treatment program for toxoplasmosis in Jilin, China.
刚地弓形虫是一种重要的人畜共患寄生虫，已在包括人类在内的许多宿主中被检测到，其广泛流行。马等动物的产品，如肉和奶，与人类生活密切相关。马等动物作为中间宿主，其在吉林省的弓形虫病情况知之甚少。因此，本研究旨在评估中国东北吉林省马等动物弓形虫感染的发生和危险因素。本研究于 2018 年 3 月至 2020 年 8 月从吉林省 6 个地区采集了 245 份马（192 匹马、25 头驴和 28 头骡子）的血液样本，并用 PCR 进行检测。采用多变量逻辑回归分析 T. gondii B1 基因的发生率，以评估与阳性率相关的危险因素。在 245 匹马中，T. gondii 分子发生率为 9.0%（22/245）。阳性率最高的是东风地区（16.3%），其次是洮南（10.0%）、汪清（8.3%）、安图（8.0%）、通化（8.0%）和舒兰（2.3%）。统计分析表明，养殖模式和地区可能是两个主要的危险因素。数据分析表明，圈养农场（3.2%，95%CI：0.0-6.7%）的阳性率明显低于散养农场（P<0.05），而舒兰地区是保护因素（OR：0.063，95%CI：0.007-0.559）。本研究结果提醒人们注意该地区马等动物弓形虫感染的存在，并为中国吉林的弓形虫病防治计划做出贡献。
