菊粉可能通过抑制肝脏中大麻素受体-1 和类脂肪酶-3 的表达来预防脂肪变性。

Inulin may prevent steatosis by suppressing cannabinoid receptor-1 and patatin-like phospholipase-3 expression in liver.

机构信息

Department of Nutrition and Dietetics, Faculty of Health Sciences, Ankara University, Ankara, Turkey.

出版信息

Nutrition. 2022 Nov-Dec;103-104:111742. doi: 10.1016/j.nut.2022.111742. Epub 2022 May 23.

DOI:10.1016/j.nut.2022.111742

PMID:35908495

Abstract

OBJECTIVES

Non-alcoholic fatty liver disease (NAFLD) is one of the major causes of liver disease worldwide. Although various molecular mechanisms are effective in the initiation and progression, the exact pathway is not completely clarified. Recent findings suggest a role of the endocannabinoid system in the pathology of NAFLD. Inulin has been shown to be beneficial for NAFLD. With the first study, we investigated the effects of inulin supplementation on NAFLD via the endocannabinoid system in Wistar rats fed high-fat diet.

METHODS

Male Wistar rats were fed with control, control plus inulin, high-fat, and high-fat plus inulin diets for 12 wk. Inulin was added to diets in 15% weight/weight. Biochemical parameters, insulin, and adiponectin levels were determined. Steatosis, lobular inflammation, and total NAFLD activity scores (NAS) were determined by histopathological analysis and by magnetic resonance imaging. Anandamide and 2-arachidonylglycerol levels were measured by the liquid chromatography-tandem mass spectrometry method. Gene expression levels were determined by the quantitative polymerase chain reaction method.

RESULTS

Our results showed that the NAS of the high-fat diet was 4.16 ± 0.30, which was significantly higher than that of the other groups. Inulin decreased Homeostasis model assessment measuring insulin resistance (HOMA-IR), serum triacylglycerol, total cholesterol, and Aspartate aminotransferaselevels. Inulin also significantly decreased Cannabinoid receptor-1 and Patatin-like phospholipase-3 gene expressions in the liver. The 2-arachidonylglycerol levels in the liver were lower in the inulin-added groups. These effects of inulin were associated with NAS.

CONCLUSIONS

Inulin prevented the development of NAFLD, possibly by affecting the expression of genes involved in the pathogenesis of NAFLD in the liver via endocannabinoids. The results of this study show that inulin may be a promising molecule in the treatment/prevention of NAFLD.

摘要

目的

非酒精性脂肪性肝病（NAFLD）是全球范围内导致肝脏疾病的主要原因之一。尽管各种分子机制在疾病的发生和发展中都有作用，但确切的途径尚未完全阐明。最近的研究结果表明，内源性大麻素系统在 NAFLD 的发病机制中起作用。菊粉已被证明对 NAFLD 有益。通过第一项研究，我们通过高脂肪饮食喂养的 Wistar 大鼠的内源性大麻素系统研究了菊粉补充剂对 NAFLD 的影响。

方法

雄性 Wistar 大鼠分别用对照、对照加菊粉、高脂肪和高脂肪加菊粉喂养 12 周。菊粉以 15%重量/重量添加到饮食中。测定生化参数、胰岛素和脂联素水平。通过组织病理学分析和磁共振成像测定脂肪变性、小叶炎症和总 NAFLD 活动评分（NAS）。通过液相色谱-串联质谱法测定大麻素受体-1 和 2-花生四烯酸甘油的水平。通过定量聚合酶链反应法测定基因表达水平。

结果

我们的结果表明，高脂肪饮食的 NAS 为 4.16±0.30，明显高于其他组。菊粉降低了稳态模型评估胰岛素抵抗（HOMA-IR）、血清三酰甘油、总胆固醇和天门冬氨酸氨基转移酶水平。菊粉还显著降低了肝脏中的大麻素受体-1 和脂肪酶样磷脂酶-3 基因的表达。添加菊粉的肝脏中 2-花生四烯酸甘油的水平较低。菊粉的这些作用与 NAS 有关。