College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
Gene. 2022 Oct 20;841:146766. doi: 10.1016/j.gene.2022.146766. Epub 2022 Jul 29.
Relatively few viruses infecting haloarchaea (haloviruses) have been reported. In this study, the genome sequence of VOLN27B, a recently described archaeal tailed virus (arTV) with a myovirus morphotype was described, along with the sequence of its host, Halorubrum spp. LN27. Halovirus VOLN27B contains a linear, dsDNA genome of 76,891 bp which is predicted to encode 109 proteins and four tRNAs (tRNA, tRNA, tRNA and tRNA). The DNA G + C content of VOLN27B genome is 56.1 mol%, nearly 10% lower than that of its host strain. A 315 bp LTR (long terminal repeat) was detected in the genome. The genome of its host strain LN27 was 3,301,211 bp (chromosome and 1 plasmid) with a DNA G + C content of 68.3 mol% and 3142 annotated protein coding genes. At least two hypothetical proviruses were detected in the genome. It lacked a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) locus. Sequence similarity and phylogenetic tree reconstructions placed it within the genus Halorubrum as a potential new species. VOLN27B exhibits a distinct difference in the frequency of codon usage against its host strain Halorubrum sp. LN27. The organization of VOLN27B genome shows remarkable synteny and amino acid sequence similarity to the genomes and predicted proteins of HF1-like haloviruses (genus Haloferacalesvirus) and a provirus in the genome of Halorubrum depositum Y78. VOLN27B and its host Halorubrum sp. LN27 comprise a new virus-host system from a hypersaline ecosystem and can be used to further understand the novel biology at extreme salt concentration.
感染盐杆菌（haloviruses）的病毒相对较少。本研究描述了一种新近描述的古菌长尾病毒（arTV）VOLN27B 的基因组序列及其宿主 Halorubrum spp. LN27 的序列。 Halovirus VOLN27B 包含一个线性、dsDNA 基因组，长度为 76891 bp，预计编码 109 种蛋白质和四个 tRNA（tRNA、tRNA、tRNA 和 tRNA）。VOLN27B 基因组的 DNA G+C 含量为 56.1 mol%，比其宿主菌株低近 10%。在基因组中检测到一个 315 bp 的 LTR（长末端重复序列）。其宿主菌株 LN27 的基因组为 3301211 bp（染色体和 1 个质粒），DNA G+C 含量为 68.3 mol%，有 3142 个注释的蛋白质编码基因。在基因组中至少检测到两个假定的前病毒。它缺乏 CRISPR（簇状规则间隔短回文重复序列）基因座。序列相似性和系统发育树重建将其置于 Halorubrum 属内，作为一种潜在的新种。VOLN27B 在密码子使用频率上与宿主菌株 Halorubrum sp. LN27 存在明显差异。VOLN27B 基因组的组织与 HF1 样盐杆菌（Haloferacalesvirus 属）和 Halorubrum depositum Y78 基因组中的一个前病毒的基因组和预测蛋白具有显著的同线性和氨基酸序列相似性。VOLN27B 及其宿主 Halorubrum sp. LN27 来自一个高盐生态系统的新型病毒-宿主系统，可以用来进一步了解极端盐浓度下的新型生物学。
