Chen Shaoxing, Dai Yongpei, Liu Jingwen, Zhang Shimin, Chen Feilong, Jin Fanjie, Ruan Peiyao, Li Lu, Chen Xiangdong
College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Archaea. 2021 Dec 14;2021:8271899. doi: 10.1155/2021/8271899. eCollection 2021.
A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (/). VOLN27B and sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.
一种新型嗜盐病毒VOLN27B是从取自中国安徽白垩纪时期形成的一层、深度约430米处的岩芯样本中分离出来的。VOLN27B感染嗜盐古菌sp. LN27，具有典型肌尾病毒的带收缩尾的头尾形态。平均头部直径为64±2.0纳米，未收缩的尾部为15±1.0×65±2.0纳米。潜伏期约为10小时。VOLN27B在sp. LN27细胞中的成熟时间接近8小时。VOLN27B在sp. LN27细胞上的吸附时间少于1分钟。病毒颗粒在pH值小于5或NaCl浓度低于12%（/）时不稳定。VOLN27B和sp. LN27是从同一高盐环境中分离得到的，为嗜盐古菌提供了一个新的病毒-宿主系统。
