Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Bioresour Technol. 2022 Oct;361:127712. doi: 10.1016/j.biortech.2022.127712. Epub 2022 Jul 28.
A bacterial image analysis system based on surface plasmon resonance imaging was established to investigate the effect of bacterial motility on biofilm formation under high ammonia nitrogen at the single-cell level. The results showed that the bacterial mean rotation speed and vertical motility distance decreased with the increasing concentration of ammonia nitrogen. Ammonia nitrogen inhibited the metabolic activity of the bacteria, decreasing bacterial motility. Bacterial motility was negatively correlated with the biofilm-formation ability. The biofilm formation ability of Enterobacter cloacae strain HNR exposed to ammonia nitrogen was enhanced by reducing its movement and promoting EPS secretion. Genes related to the tricarboxylic acid cycle and oxidative phosphorylation were down-regulated, indicating inhibition of microbial energy metabolism. Genes related to bacterial secretion and lipopolysaccharide synthesis were up-regulated, facilitating the formation of biofilms and enabling the bacteria to resist ammonia nitrogen stress. This study provides new insights into the biofilm formation under ammonia stress.
建立了基于表面等离子体共振成像的细菌图像分析系统，以在单细胞水平上研究高氨氮条件下细菌运动对生物膜形成的影响。结果表明，细菌的平均旋转速度和垂直运动距离随氨氮浓度的增加而降低。氨氮抑制了细菌的代谢活性，降低了细菌的运动能力。细菌的运动能力与生物膜形成能力呈负相关。通过降低运动能力和促进 EPS 分泌，暴露于氨氮的阴沟肠杆菌 HNR 菌株的生物膜形成能力得到增强。与三羧酸循环和氧化磷酸化相关的基因下调，表明微生物能量代谢受到抑制。与细菌分泌和脂多糖合成相关的基因上调，有利于生物膜的形成，并使细菌能够抵抗氨氮胁迫。本研究为氨胁迫下的生物膜形成提供了新的见解。
