人成骨细胞对不同牙种植体基台材料的反应：一项体外研究。

Human osteoblasts response to different dental implant abutment materials: An in-vitro study.

机构信息

Division of Dentistry, School of Medical Sciences, University of Manchester, Coupland 3 Building, Oxford Road, Manchester M13 9PL, United Kingdom; Periodontology Department, Faculty of Dentistry, The University of Benghazi, Benghazi, Libya; Restorative Department, Faculty of Dentistry, Libyan International Medical University, Benghazi, Libya; Blond McIndoe Laboratories, Division of Cell Matrix Biology & Regenerative Medicine, Faculty of Biology, Medicine & Health, The University of Manchester, 3.106 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.

Prosthodontic Department, School of Dentistry, University of Jordan, Amman, Jordan.

出版信息

Dent Mater. 2022 Sep;38(9):1547-1557. doi: 10.1016/j.dental.2022.07.005. Epub 2022 Jul 29.

DOI:10.1016/j.dental.2022.07.005

PMID:35909000

Abstract

OBJECTIVES

This study aimed to investigate human osteoblasts (HOB) response towards different dental implant abutment materials.

METHODS

Five dental implant abutment materials were investigated: (1) titanium (Ti), (2) titanium coated nitride (TiN), (3) cobalt chromium (CoCr), (4) zirconia (ZrO₂), and (5) modified polyether ether ketone (m-PEEK). HOBs were cultured, expanded, and seeded according to the supplier's protocol (PromoCell, UK). Cell proliferation and cytotoxicity were evaluated at days 1, 3, 5, and 10 using Alamar Blue (alamarBlue) and lactate dehydrogenase (LDH) colorimetric assays. Data were analysed via two-way ANOVA, one-way ANOVA and Tukey's post hoc test (significance was determined as p < 0.05 for all tests).

RESULTS

All the investigated materials showed high and comparable initial proliferation activities apart from ZrO₂ (46.92%), with P% of 79.91%, 68.77%, 73.20%, and 65.46% for Ti, TiN, CoCr, and m-PEEK, respectively. At day 10, all materials exhibited comparable and lower P% than day 1 apart from TiN (70.90%) with P% of 30.22%, 40.64%, 37.27%, and 50.65% for Ti, CoCr, ZrO₂, and m-PEEK, respectively. The cytotoxic effect of the investigated materials was generally low throughout the whole experiment. At day 10, the cytotoxicity % was 7.63%, 0.21%, 13.30%, 5.32%, 8.60% for Ti, TiN, CoCr, ZrO₂, and m-PEEK. The Two-way ANOVA and Tukey's Multiple Comparison Method highlighted significant material and time effects on cell proliferation and cytotoxicity, and a significant interaction (p < 0.0001) between the tested materials. Notably, TiN and m-PEEK showed improved HOB proliferation activity and cytotoxic levels than the other investigated materials. In addition, a non-significant negative correlation between viability and cytotoxicity was found for all tested materials. Ti (p = 0.07), TiN (p = 0.28), CoCr (p = 0.15), ZrO₂ (p = 0.17), and m-PEEK (p = 0.12).

SIGNIFICANCE

All the investigated materials showed excellent biocompatibility properties with more promising results for the newly introduced TiN and m-PEEK as alternatives to the traditionally used dental implant and abutment materials.

摘要

目的

本研究旨在探究不同牙科种植体基台材料对人成骨细胞（HOB）的反应。

方法

研究了 5 种牙科种植体基台材料：（1）钛（Ti），（2）氮化钛（TiN），（3）钴铬（CoCr），（4）氧化锆（ZrO₂）和（5）改性聚醚醚酮（m-PEEK）。HOB 按照供应商的方案进行培养、扩增和接种（PromoCell，英国）。使用 Alamar Blue（alamarBlue）和乳酸脱氢酶（LDH）比色法在第 1、3、5 和 10 天评估细胞增殖和细胞毒性。通过双因素方差分析、单因素方差分析和 Tukey 事后检验对数据进行分析（所有检验的显著性水平均定为 p < 0.05）。

结果

除 ZrO₂（46.92%）外，所有研究材料的初始增殖活性均较高且相当，Ti、TiN、CoCr 和 m-PEEK 的 P%分别为 79.91%、68.77%、73.20%和 65.46%。第 10 天，与第 1 天相比，所有材料的 P%均较低，除 TiN（70.90%）外，Ti、CoCr、ZrO₂和 m-PEEK 的 P%分别为 30.22%、40.64%、37.27%和 50.65%。整个实验过程中，研究材料的细胞毒性一般较低。第 10 天，细胞毒性%分别为 Ti、TiN、CoCr、ZrO₂和 m-PEEK 的 7.63%、0.21%、13.30%、5.32%和 8.60%。双因素方差分析和 Tukey 多重比较法强调了材料和时间对细胞增殖和细胞毒性的显著影响，以及测试材料之间的显著相互作用（p < 0.0001）。值得注意的是，TiN 和 m-PEEK 显示出比其他研究材料更高的 HOB 增殖活性和细胞毒性水平。此外，所有测试材料的存活率与细胞毒性之间呈非显著负相关。Ti（p = 0.07）、TiN（p = 0.28）、CoCr（p = 0.15）、ZrO₂（p = 0.17）和 m-PEEK（p = 0.12）。