Osman Muataz A, Kushnerev Evgeny, Alamoush Rasha A, Seymour Kevin G, Yates Julian M
Division of Dentistry, School of Medical Sciences, Coupland 3 Building, University of Manchester, Oxford Road, Manchester M13 9PL, UK.
Periodontology Department, Faculty of Dentistry, The University of Benghazi, Benghazi, Libya.
Dent J (Basel). 2022 Oct 17;10(10):192. doi: 10.3390/dj10100192.
Objectives: This study aimed to investigate the response of human gingival fibroblasts (HGFB) and human gingival keratinocytes (HGKC) towards different dental implant abutment materials. Methods: Five materials were investigated: (1) titanium (Ti), (2) titanium nitride (TiN), (3) cobalt-chromium (CoCr), (4) zirconia (ZrO2), and (5) modified polyether ether ketone (m-PEEK). Both cell lines were cultured, expanded, and seeded in accordance with the protocol of their supplier. Cell proliferation and cytotoxicity were evaluated at days 1, 3, 5, and 10 using colourimetric viability and cytotoxicity assays. Data were analysed via two-way ANOVA, one-way ANOVA, and Tukey’s post hoc test (p < 0.05 for all tests). Results: There was a statistically significant difference in cell proliferation of HGKC and HGFB cells in contact with different abutment materials at different time points, with no significant interaction between different materials. There was a significant effect on cell proliferation and cytotoxicity with different exposure times (p < 0.0001) for each material. Cell proliferation rates were comparable for both cell lines at the beginning of the study, however, HGFB showed higher proliferation rates for all materials at day 10 with better proliferation activities with ZrO and m-PEEK (40.27%) and (48.38%) respectively. HGKC showed significant interactions (p < 0.0001) in cytotoxicity between different materials. Conclusion: The present in vitro assessment investigated the biocompatibility of different abutment materials with soft tissue cells (HGFB and HGKC). The findings suggest that m-PEEK and TiN are biologically compatible materials with human cells that represent the soft tissue and can be considered as alternative implant abutment materials to Ti and ZrO2, especially when the aesthetic is of concern.
本研究旨在调查人牙龈成纤维细胞（HGFB）和人牙龈角质形成细胞（HGKC）对不同牙种植体基台材料的反应。方法：研究了五种材料：（1）钛（Ti），（2）氮化钛（TiN），（3）钴铬合金（CoCr），（4）氧化锆（ZrO₂），以及（5）改性聚醚醚酮（m-PEEK）。两种细胞系均按照供应商的方案进行培养、扩增和接种。使用比色法活力和细胞毒性测定法在第1、3、5和10天评估细胞增殖和细胞毒性。数据通过双向方差分析、单向方差分析和Tukey事后检验进行分析（所有检验p < 0.05）。结果：在不同时间点，与不同基台材料接触的HGKC和HGFB细胞的细胞增殖存在统计学显著差异，不同材料之间无显著相互作用。每种材料在不同暴露时间对细胞增殖和细胞毒性有显著影响（p < 0.0001）。在研究开始时，两种细胞系的细胞增殖率相当，然而，在第10天，HGFB对所有材料的增殖率更高，对ZrO和m-PEEK的增殖活性更好，分别为（40.27%）和（48.38%）。HGKC在不同材料之间的细胞毒性方面表现出显著相互作用（p < 0.0001）。结论：本体外评估研究了不同基台材料与软组织细胞（HGFB和HGKC）的生物相容性。研究结果表明，m-PEEK和TiN是与代表软组织的人体细胞具有生物相容性的材料，可被视为Ti和ZrO₂的替代种植体基台材料，特别是在美观方面值得关注时。
