hsa_circ_0084811 通过 miR-18a-5p/miR-18b-5p/E2F5 轴调控视网膜母细胞瘤细胞增殖和凋亡。
hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis.
机构信息
Department of Ophthalmology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, China.
Hemodialysis Room, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, China.
出版信息
Biomed Res Int. 2022 Jul 19;2022:6918396. doi: 10.1155/2022/6918396. eCollection 2022.
BACKGROUND
Retinoblastoma (RB) is the commonest primary intraocular malignancy during childhood. Circular RNAs (circRNAs) act as regulators in RB development, and hsa_circ_E2F5 (circ_0084811 in this study) was found to be highly expressed in RB cells, so we wanted to identify its detailed molecular mechanism.
METHODS
The expression level of circ_0084811 in RB cells was tested by RT-qPCR and its effects on RB cells were evaluated through functional assays. The regulatory mechanism that circ_0084811 may exert in RB progression was testified through mechanism experiments.
RESULTS
High circ_0084811 expression in RB cells facilitated cell proliferation but inhibited cell apoptosis. The enrichment of acetylation of histone 3 lysine 27 (H3K27ac) in circ_0084811 promoter induced circ_0084811 upregulation. Moreover, circ_0084811 regulated E2F transcription factor 5 (E2F5) expression via sponging microRNA-18a-5p (miR-18a-5p) and microRNA-18b-5p (miR-18b-5p).
CONCLUSION
circ_0084811 modulated RB progression via the miR-18a-5p/miR-18b-5p/E2F5 axis.
背景
视网膜母细胞瘤（RB）是儿童期最常见的眼内原发性恶性肿瘤。环状 RNA（circRNAs）在 RB 发育中起调节作用，我们发现 hsa_circ_E2F5（本研究中的 circ_0084811）在 RB 细胞中高度表达，因此我们想确定其详细的分子机制。
方法
通过 RT-qPCR 检测 RB 细胞中 circ_0084811 的表达水平，并通过功能测定评估其对 RB 细胞的影响。通过机制实验验证 circ_0084811 可能在 RB 进展中发挥的调节机制。
结果
RB 细胞中 circ_0084811 的高表达促进了细胞增殖，但抑制了细胞凋亡。circ_0084811 启动子中组蛋白 3 赖氨酸 27 乙酰化（H3K27ac）的富集诱导 circ_0084811 的上调。此外，circ_0084811 通过海绵 microRNA-18a-5p（miR-18a-5p）和 microRNA-18b-5p（miR-18b-5p）调节 E2F 转录因子 5（E2F5）的表达。
结论
circ_0084811 通过 miR-18a-5p/miR-18b-5p/E2F5 轴调节 RB 的进展。
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