Yang Jing, Duan Jie, Li Meifang, Sun Haidong, Sun Yongsheng, Pan Wei, Xi Haixiang
Department of Encephalopathy, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, China.
Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, China.
Contrast Media Mol Imaging. 2022 Jul 15;2022:8199917. doi: 10.1155/2022/8199917. eCollection 2022.
To investigate the prognostic potential of serum aldehyde dehydrogenase isoform 1 (ALDH1) level in acute cerebral infarction, and the molecular mechanism in mediating neurological deficits, a total of 120 acute cerebral infarction cases within 72 h of onset were retrospectively analyzed. Serum ALDH1 level in them was detected by qRT-PCR. Receiver operating characteristic (ROC) and Kaplan-Meier curves were depicted for assessing the diagnostic and prognostic potentials of ALDH1 in acute cerebral infarction, respectively. An acute cerebral infarction model in rats was established by performing MCAO, followed by evaluation of neurological deficits using mNSS and detection of relative levels of ALDH1, Smad2, Smad4, and p21 in rat brain tissues. Pearson's correlation test was carried out to verify the correlation between ALDH1 and mNSS and relative levels of Smad2, Smad4, and p21. Serum ALDH1 level increased in acute cerebral infarction patients. A high level of ALDH1 predicted a poor prognosis of acute cerebral infarction patients. In addition, ALDH1 was sensitive and specific in distinguishing acute cerebral infarction cases, presenting a certain diagnostic potential. mNSS was remarkably higher in acute cerebral infarction rats than that of controls. Compared with sham operation group, relative levels of ALDH1, Smad2, and Smad4 were higher in brain tissues of modeling rats, whilst p21 level was lower. ALDH1 level in brain tissues of modeling rats was positively correlated to mNSS, and mRNA levels of Smad2 and Smad4, but negatively correlated to p21 level. Serum ALDH1 level is a promising prognostic and diagnostic factor of acute cerebral infarction, which is correlated to 90-day mortality. Increased level of ALDH1 in the brain of cerebral infarction rats is closely linked to neurological function, which is associated with the small mothers against decapentaplegic (Smad) signaling and p21.
为探讨血清醛脱氢酶同工酶1（ALDH1）水平对急性脑梗死的预后评估价值及其介导神经功能缺损的分子机制，回顾性分析120例发病72小时内的急性脑梗死患者。采用qRT-PCR检测其血清ALDH1水平。绘制受试者工作特征（ROC）曲线和Kaplan-Meier曲线，分别评估ALDH1对急性脑梗死的诊断和预后价值。通过大脑中动脉闭塞（MCAO）建立大鼠急性脑梗死模型，采用改良神经功能缺损评分（mNSS）评估神经功能缺损，检测大鼠脑组织中ALDH1、Smad2、Smad4和p21的相对水平。采用Pearson相关性检验验证ALDH1与mNSS及Smad2、Smad4和p21相对水平之间的相关性。急性脑梗死患者血清ALDH1水平升高。高水平的ALDH1预示急性脑梗死患者预后不良。此外，ALDH1在鉴别急性脑梗死病例方面具有敏感性和特异性，具有一定的诊断价值。急性脑梗死大鼠的mNSS显著高于对照组。与假手术组相比，模型大鼠脑组织中ALDH1、Smad2和Smad4的相对水平升高，而p21水平降低。模型大鼠脑组织中ALDH1水平与mNSS、Smad2和Smad4的mRNA水平呈正相关，与p21水平呈负相关。血清ALDH1水平是急性脑梗死有前景的预后和诊断指标，与90天死亡率相关。脑梗死大鼠脑内ALDH1水平升高与神经功能密切相关，其与小母亲抗五肢瘫（Smad）信号通路和p21有关。
