Shurigin Vyacheslav, Alimov Jakhongir, Davranov Kakhramon, Gulyamova Tashkhan, Egamberdieva Dilfuza
Faculty of Biology, National University of Uzbekistan, Tashkent, 100174, Uzbekistan.
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Tashkent, 100128, Uzbekistan.
Curr Res Microb Sci. 2022 Apr 12;3:100133. doi: 10.1016/j.crmicr.2022.100133. eCollection 2022.
This study reports the diversity of cultivable endophytic bacteria associated with yellow iris ( L.) by using 16S rRNA gene analysis and their plant beneficial traits. The 16S rRNA sequence similarities of endophytic bacteria isolated from the leaves and roots of yellow iris showed that the isolates belonged to the genera and . The endophytic bacteria HRT18, HRT8, HRT13, and HST13 exhibited antimicrobial activity against five plant pathogenic fungi and . They also demonstrated the capability to produce chitinase, protease, glucanase, lipase, HCN, and indole-3-acetic acid (IAA). Thirteen isolates (46%) produced IAA, and the most active IAA producers were , and . The IAA producing bacterial isolates stimulated root and shoot growth of garden cress. Our findings suggest that medicinal plants could be a promising source for isolating plant-beneficial bacteria that can be used to enhance the growth and protect plants against soil-borne pathogens.
本研究通过16S rRNA基因分析报告了与黄菖蒲(Iris pseudacorus L.)相关的可培养内生细菌的多样性及其植物有益特性。从黄菖蒲叶片和根部分离出的内生细菌的16S rRNA序列相似性表明,分离物属于 属和 属。内生细菌HRT18、HRT8、HRT13和HST13对五种植物病原真菌 和 表现出抗菌活性。它们还表现出产生几丁质酶、蛋白酶、葡聚糖酶、脂肪酶、HCN和吲哚-3-乙酸(IAA)的能力。13株分离物(46%)产生IAA,最活跃的IAA产生菌是 、 和 。产生IAA的细菌分离物刺激了水芹的根和芽生长。我们的研究结果表明,药用植物可能是分离植物有益细菌的一个有前景的来源,这些细菌可用于促进植物生长并保护植物免受土传病原体的侵害。