Shurigin Vyacheslav, Alaylar Burak, Davranov Kakhramon, Wirth Stephan, Bellingrath-Kimura Sonoko Dorothea, Egamberdieva Dilfuza
Department of Microbiology and Biotechnology, Faculty of Biology, National University of Uzbekistan, 100174, Tashkent, Uzbekistan.
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, 100128 Tashkent, Uzbekistan.
AIMS Microbiol. 2021 Sep 13;7(3):336-353. doi: 10.3934/microbiol.2021021. eCollection 2021.
Endophytes colonizing plant tissue play an essential role in plant growth, development, stress tolerance and plant protection from soil-borne diseases. In this study, we report the diversity of cultivable endophytic bacteria associated with marigold ( L.) by using 16S rRNA gene analysis and their plant beneficial properties. A total of 42 bacterial isolates were obtained from plant tissues of marigold. They belonged to the genera , and . Among the bacterial strains, FRT12, and FST5 showed moderate or vigorous inhibition against three tested plant pathogenic fungi, and . They also demonstrated the capability to produce hydrolytic enzymes and indole-3-acetic acid (IAA). Five out of 16 isolates significantly stimulated shoot and root growth of marigold in a pot experiment. The present study reveals that more than half of the bacterial isolates associated with marigold ( L.) provided antifungal activity against one or more plant pathogenic fungi. Our findings suggest that medicinal plants with antimicrobial activity could be a source for selecting microbes with antagonistic activity against fungal plant pathogens or with plant growth stimulating potential. These isolates might be considered as promising candidates for the improvement of plant health.
定殖于植物组织中的内生菌在植物生长、发育、胁迫耐受性以及植物抵御土传病害方面发挥着重要作用。在本研究中，我们通过16S rRNA基因分析报告了与万寿菊（Tagetes erecta L.）相关的可培养内生细菌的多样性及其对植物有益的特性。从万寿菊的植物组织中总共获得了42株细菌分离物。它们属于芽孢杆菌属（Bacillus）、假单胞菌属（Pseudomonas）和伯克霍尔德菌属（Burkholderia）。在这些细菌菌株中，芽孢杆菌属菌株FRT12、伯克霍尔德菌属菌株FST5对三种测试的植物病原真菌（立枯丝核菌（Rhizoctonia solani）、尖孢镰刀菌（Fusarium oxysporum）和终极腐霉（Pythium ultimum））表现出中度或强烈的抑制作用。它们还表现出产生水解酶和吲哚-3-乙酸（IAA）的能力。在盆栽试验中，16株分离物中有5株显著促进了万寿菊地上部和根部的生长。本研究表明，与万寿菊（Tagetes erecta L.）相关的细菌分离物中，超过一半对一种或多种植物病原真菌具有抗真菌活性。我们的研究结果表明，具有抗菌活性的药用植物可能是筛选对植物病原真菌具有拮抗活性或具有植物生长刺激潜力的微生物的来源。这些分离物可能被视为改善植物健康的有前途的候选者。
