Meethangdee Mathurin, Pathom-Aree Wasu
Multidisciplinary and Interdisciplinary School, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Excellence in Microbial Diversity and Sustainable Utilization, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Curr Res Microb Sci. 2024 Oct 1;7:100284. doi: 10.1016/j.crmicr.2024.100284. eCollection 2024.
Bryophytes are non-vascular plants with dominant gametophyte stage that play vital ecological roles in natural ecosystems. Unfortunately, their populations are currently in decline due to habitat destruction and various anthropogenic activities. The conservation efforts for bryophytes are hampered by their slow growth rates. This study aims to investigate the potential of actinobacteria to promote the growth of bryophytes. In this study, three plant growth-promoting actinobacteria, MT1.1, CMU55-4 and S3 were cultured in International Project medium 2 (ISP2) broth to obtain culture filtrates containing bioactive compounds for enhancing the growth of two bryophyte species, (C. Ludw.) Fürnr and C. Müll. Interestingly, the incorporation of actinobacterial culture filtrates into 1/16 Murashige and Skoog (MS) medium yielded superior growth performance of (C. Ludw.) Fürnr and C. Müll, as observed from the thallus height, fresh weight, total chlorophyll contents, and total carotenoid contents compared to control groups. In addition, the inoculation of CMU55-4 on C. Müll grown in sterile peat moss demonstrated the highest values for thallus height, fresh weight, dry weight, total chlorophyll content, and total carotenoid content. All actinobacteria successfully colonized the moss seedlings without any observable negative impacts, indicating beneficial interactions between actinobacteria and bryophytes. This research sheds light on the potential of harnessing plant beneficial actinobacteria to enhance the growth of bryophytes for conservation purposes.
苔藓植物是配子体占主导阶段的非维管植物，在自然生态系统中发挥着至关重要的生态作用。不幸的是，由于栖息地破坏和各种人为活动，它们的种群数量目前正在减少。苔藓植物生长速度缓慢，这阻碍了其保护工作。本研究旨在调查放线菌促进苔藓植物生长的潜力。在本研究中，将三种促进植物生长的放线菌MT1.1、CMU55 - 4和S3在国际项目培养基2（ISP2）肉汤中培养，以获得含有生物活性化合物的培养滤液，用于促进两种苔藓植物（C. Ludw.）Fürnr和C. Müll的生长。有趣的是，与对照组相比，从叶状体高度、鲜重、总叶绿素含量和总类胡萝卜素含量来看，将放线菌培养滤液加入1/16的Murashige和Skoog（MS）培养基中，（C. Ludw.）Fürnr和C. Müll的生长表现更佳。此外，在无菌泥炭藓中生长的C. Müll上接种CMU55 - 4，其叶状体高度、鲜重、干重、总叶绿素含量和总类胡萝卜素含量均达到最高值。所有放线菌都成功地在苔藓幼苗上定殖，没有任何明显的负面影响，这表明放线菌与苔藓植物之间存在有益的相互作用。这项研究揭示了利用对植物有益的放线菌来促进苔藓植物生长以进行保护的潜力。
