经鼻应用的人嗅黏膜神经祖细胞迁移至受损脑区。
Intranasally applied human olfactory mucosa neural progenitor cells migrate to damaged brain regions.
作者信息
Kronner John M, Folbe Adam, Meythaler Jay, Nelson John O, Borisov Andrei, Peduzzi Jean D
机构信息
Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Department of Otolaryngology, Oakland University William Beaumont School of Medicine, Rochester, MI 48309, USA.
出版信息
Future Sci OA. 2022 Jul 12;8(6):FSO806. doi: 10.2144/fsoa-2022-0012. eCollection 2022 Jul.
AIM
To determine if intranasally administered olfactory mucosa progenitor cells (OMPCs) migrate to damaged areas of brain.
MATERIALS & METHODS: Rowett Nude (RNU) adult rats were injured using the Marmarou model then 2 weeks later received intranasally-delivered human OMPC. After 3 weeks, rats were sacrificed and brain sectioned. The mean distances from the human OMPCs to markers for degenerative neuronal cell bodies (p-c-Jun), axonal swellings on damaged axons (β-APP) and random points in immunostained sections were quantified. One-way ANOVA was used to analyze data.
RESULTS
The human OMPCs were seen in specific areas of the brain near degenerating cell bodies and damaged axons.
CONCLUSION
Intranasally delivered human OMPC selectively migrate to brain injury sites suggesting a possible noninvasive stem cell delivery for brain injury.
目的
确定经鼻给药的嗅黏膜祖细胞（OMPCs）是否迁移至脑损伤区域。
材料与方法
采用 Marmarou 模型损伤成年 Rowett 裸鼠（RNU），2 周后经鼻给予人 OMPC。3 周后，处死大鼠并对脑进行切片。对人 OMPCs 到退行性神经元细胞体标志物（p-c-Jun）、受损轴突上的轴突肿胀（β-APP）以及免疫染色切片中的随机点的平均距离进行量化。采用单因素方差分析来分析数据。
结果
在靠近退行性细胞体和受损轴突的脑特定区域观察到了人 OMPCs。
结论
经鼻给予的人 OMPC 选择性迁移至脑损伤部位，提示可能存在一种用于脑损伤的非侵入性干细胞递送方式。
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