Ladikan Olga, Silyavka Elena, Mitrofanov Andrei, Laptenkova Anastasia, Shilovskikh Vladimir, Kolonitckii Petr, Ivanov Nikita, Remezov Andrey, Fedorova Anna, Khripun Vassily, Pestova Olga, Podolskaya Ekaterina P, Sukhodolov Nikolai G, Selyutin Artem A
St. Petersburg State University, Universitetskaya emb. 7-9, 199034 St. Petersburg, Russia.
Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Str. 6, 01069 Dresden, Germany.
ACS Omega. 2022 Jul 14;7(29):24973-24981. doi: 10.1021/acsomega.1c07300. eCollection 2022 Jul 26.
This article presents new possibilities of using thin films of lanthanide stearates as sorbent materials. Modification of the Q-sense device resonator with monolayers of lanthanide stearates by the Langmuir-Schaeffer method made it possible to study the process of insulin protein adsorption on the surface of new thin-film sorbents. The resulting films were also characterized by compression isotherms, chemical analysis, scanning electron microscopy, and mass spectrometry. The transition of stearic acid to salt was recorded by IR spectroscopy. Using the LDI MS method, the main component of thin films, lanthanide distearate, was established. The presence of Eu in thin films was revealed. In the case of europium stearate, the maximum value of insulin adsorption was obtained, -1.67·10 mole/cm. The findings suggest the possibility of using thin films of lanthanide stearates as a sorption material for the proteomics determination of the quantitative protein content in complex fluid systems by specific adsorption on modified surfaces and isolation of such proteins from complex mixtures.
本文介绍了使用硬脂酸镧薄膜作为吸附材料的新可能性。通过朗缪尔-谢弗法用硬脂酸镧单分子层对Q-传感装置谐振器进行修饰，使得研究胰岛素蛋白在新型薄膜吸附剂表面的吸附过程成为可能。所得薄膜还通过压缩等温线、化学分析、扫描电子显微镜和质谱进行了表征。通过红外光谱记录了硬脂酸向盐的转变。使用激光解吸电离质谱法确定了薄膜的主要成分——二硬脂酸镧。揭示了薄膜中铕的存在。在硬脂酸铕的情况下，获得了胰岛素吸附的最大值，即-1.67·10摩尔/平方厘米。这些发现表明，硬脂酸镧薄膜有可能作为一种吸附材料，用于通过在修饰表面上的特异性吸附以及从复杂混合物中分离此类蛋白质，对复杂流体系统中的蛋白质含量进行蛋白质组学定量测定。
