Zhu Di-Hua, Nie Fang-Hong, Zhang Min, Wei Wan, Song Qing-Lang, Hu Yao, Kang Dan-Ju, Chen Zhi-Bao, Lin Hong-Ying, Chen Jin-Jun
Department of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, China.
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China.
Front Nutr. 2022 Jul 13;9:929925. doi: 10.3389/fnut.2022.929925. eCollection 2022.
Polychlorinated biphenyls (PCBs) are widely distributed environmental toxicants, whose biological toxicity is magnified step by step through the transmission of the food chain. However, there is little research about the effect of PCBs on intestinal epithelial barrier function. In this experiment, the effects of PCB exposure on the intestines of zebrafish were evaluated. Animals were exposed to Aroclor 1254 (5 μg/L, 10 μg/L, 15 μg/L). After 21 days, the changes in histology, enzyme biomarkers, intestinal microorganisms, and metabolomics were detected. The inflammation and oxidative stress in the intestines of zebrafish were observed. Additionally, there were significant changes in intestinal microbiota and tissue metabolism, most of which were associated with oxidative stress, inflammation, and lipid metabolism. The results showed that PCBs exposure resulted in intestinal inflammation and oxidative stress in zebrafish.Moreover, intestinal metabolites and intestinal microflora of zebrafish were also disturbed. This study verified that exposure can lead to intestinal damage and changes in intestinal metabolic capacity and microorganisms, enlightening the consequences of PCB exposure.
多氯联苯（PCBs）是广泛分布的环境毒物，其生物毒性通过食物链传递而逐步放大。然而，关于多氯联苯对肠道上皮屏障功能影响的研究较少。在本实验中，评估了多氯联苯暴露对斑马鱼肠道的影响。将动物暴露于Aroclor 1254（5μg/L、10μg/L、15μg/L）。21天后，检测组织学、酶生物标志物、肠道微生物群和代谢组学的变化。观察斑马鱼肠道中的炎症和氧化应激。此外，肠道微生物群和组织代谢有显著变化，其中大多数与氧化应激、炎症和脂质代谢有关。结果表明，多氯联苯暴露导致斑马鱼肠道炎症和氧化应激。此外，斑马鱼的肠道代谢物和肠道微生物群也受到干扰。本研究证实，暴露可导致肠道损伤以及肠道代谢能力和微生物的变化，揭示了多氯联苯暴露的后果。
