State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.
Sci Total Environ. 2019 Apr 20;662:246-253. doi: 10.1016/j.scitotenv.2019.01.245. Epub 2019 Jan 22.
Microplastics (MPs) can be ingested by a variety of species and mainly accumulate in the gut. However, the consequences of MPs exposure in the gut are largely unknown. Here we evaluated the impacts of MPs exposure in zebrafish gut. Animals were experimentally exposed to polystyrene MPs (5-μm beads; 50 μg/L and 500 μg/L) for 21 days and monitored for alterations in tissue histology, enzymatic biomarkers, gut microbiome and metabolomic responses. Inflammation and oxidative stress were observed in the zebrafish gut after exposed to MPs. Furthermore, significant alterations in the gut microbiome and tissue metabolic profiles were observed, with most of these were associated with oxidative stress, inflammation and lipid metabolism. This study provides evidence that MPs exposure causes gut damage as well as alterations in gut metabolome and microbiome, yielding novel insights into the consequences of MPs exposure.
微塑料（MPs）可以被多种物种摄入，并主要在肠道中积累。然而，MPs 暴露在肠道中的后果在很大程度上是未知的。在这里，我们评估了 MPs 暴露在斑马鱼肠道中的影响。动物被实验性地暴露于聚苯乙烯 MPs（5-μm 珠；50μg/L 和 500μg/L）21 天，并监测组织组织学、酶生物标志物、肠道微生物组和代谢组学反应的变化。在 MPs 暴露后，斑马鱼肠道中观察到炎症和氧化应激。此外，还观察到肠道微生物组和组织代谢谱的显著改变，其中大多数与氧化应激、炎症和脂质代谢有关。这项研究提供了证据，表明 MPs 暴露会导致肠道损伤，以及肠道代谢组和微生物组的改变，为 MPs 暴露的后果提供了新的见解。
