Monti M, Hedner P, Ikomi-Kumm J, Valdemarsson S
Acta Endocrinol (Copenh). 1987 May;115(1):87-90.
Erythrocyte metabolism was studied in vitro by microcalorimetry in 10 hyperthyroid subjects before and after treatment. By inhibiting the enzyme enolase in the Embden-Meyerhof pathway with sodium fluoride (NaF) we have recorded the anaerobic and aerobic contributions in erythrocyte thermogenesis. The decrease in heat production rate in samples with NaF corresponds to the anaerobic contribution, whereas the values from samples with NaF reflect aerobic processes. Before treatment, total heat production rate was 120 +/- 2 mW/l erythrocytes which was higher than the post-treatment value of 99 +/- 2 (P less than 0.001) as well as the value for 14 euthyroid subjects, 108 +/- 2 mW/l (P less than 0.001). The NaF inhibitable rate was 73 +/- 2 before and 63 +/- 1 mW/l after therapy (P less than 0.01). These values correspond to 61 +/- 1 and 64 +/- 1% (n.s.) of the total heat production rate, and were similar to that of 61 +/- 2% for the controls. Heat production rates in the presence of NaF were 47 +/- 1 before and 36 +/- 1 mW/l after therapy (P less than 0.001), representing 39 +/- 1 and 36 +/- 1% of total values, respectively. The present results show that overall metabolism is increased in erythrocytes from hyperthyroid subjects before treatment and returns to normal after normalization of the thyroid function. Moreover, by using microcalorimetry we found that the metabolic activity along the Embden-Meyerhof anaerobic pathway as well as along the hexose monophosphate aerobic pathway in erythrocytes is stimulated by thyroid hormones.
通过微量量热法对10例甲状腺功能亢进患者治疗前后的红细胞代谢进行了体外研究。用氟化钠（NaF）抑制糖酵解途径中的烯醇化酶，我们记录了红细胞产热中的无氧和有氧贡献。添加NaF的样品中产热率的降低对应于无氧贡献，而添加NaF的样品的值反映有氧过程。治疗前，总产热率为120±2 mW/l红细胞，高于治疗后的值99±2（P<0.001）以及14例甲状腺功能正常受试者的值108±2 mW/l（P<0.001）。治疗前NaF可抑制率为73±2，治疗后为63±1 mW/l（P<0.01）。这些值分别占总产热率的61±1%和64±1%（无显著差异），与对照组的61±2%相似。添加NaF时的产热率治疗前为47±1，治疗后为36±1 mW/l（P<0.001），分别占总值的39±1%和36±1%。目前的结果表明，甲状腺功能亢进患者治疗前红细胞的整体代谢增加，甲状腺功能正常后恢复正常。此外，通过微量量热法我们发现，甲状腺激素可刺激红细胞中沿糖酵解无氧途径以及磷酸己糖有氧途径的代谢活性。
