Department of Neurosurgery, Affiliated Hospital of Qingdao University, Qingdao 266000, China; Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao 266000, China.
Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao 266000, China.
Biomater Adv. 2022 Jul;138:212964. doi: 10.1016/j.bioadv.2022.212964. Epub 2022 Jun 2.
There is an increasing demand for biomaterials with skull regeneration for clinical application. However, most of the current skull repair materials still have limitations, such as inadequate sources, poor cell adherence, differentiation, tissue infiltration, and foreign body sensation. Therefore, this study developed porous microsphere-based scaffolds containing mouse embryonic osteoblast precursor cells (MC3T3-E1 cells) and calcitriol (Cal) using gelatin and gelatin/hydroxyapatite through green freeze-crosslinking and freeze-drying. Gelatin was employed to prepare porous microspheres with a particle size of 100-300 μm, containing open pores of 2-70 μm and interconnected paths. Furthermore, the addition of Cal to porous gelatin microsphere-based scaffolds containing MC3T3-E1 cells (PGMSs-MC) and porous gelatin/hydroxyapatite composite microspheres containing MC3T3-E1 cells (HPGMSs-MC) improved their osteoinductivity and cell proliferation and promoted the formation of mature and well-organized bone. The developed Cal-HPGMSs-MC and Cal-PGMSs-MC displayed a good porous structure and cytocompatibility, histocompatibility, osteoconductivity, and osteoinduction. Thus, the designed scaffolds provide a promising prospect for tissue-engineered constructs with skull growth and integration, laying a foundation for further research on the reconstruction of skull defects.
对于可用于临床应用的具有头骨再生功能的生物材料的需求日益增长。然而，目前大多数的头骨修复材料仍然存在一些局限性，例如来源不足、细胞黏附性差、分化能力差、组织浸润性差和异物感强等。因此，本研究使用明胶和明胶/羟基磷灰石通过绿色冷冻交联和冷冻干燥的方法，开发了含有鼠胚胎成骨前体细胞（MC3T3-E1 细胞）和骨化三醇（Cal）的多孔微球支架。明胶用于制备粒径为 100-300 μm 的多孔微球，其含有 2-70 μm 的开放孔和相互连接的路径。此外，将 Cal 添加到含有 MC3T3-E1 细胞的多孔明胶微球支架（PGMSs-MC）和含有 MC3T3-E1 细胞的多孔明胶/羟基磷灰石复合微球（HPGMSs-MC）中，提高了它们的成骨诱导能力和细胞增殖能力，并促进了成熟和组织有序的骨形成。所开发的 Cal-HPGMSs-MC 和 Cal-PGMSs-MC 具有良好的多孔结构和细胞相容性、组织相容性、骨传导性和骨诱导性。因此，设计的支架为具有头骨生长和整合功能的组织工程构建体提供了广阔的前景，为进一步研究颅骨缺损的重建奠定了基础。
