School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.
School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.
Carbohydr Polym. 2023 Jan 1;299:120188. doi: 10.1016/j.carbpol.2022.120188. Epub 2022 Oct 5.
Most bone repair scaffolds are multi-connected channel structure, but the hollow structure is not conducive to the transmission of active factors, cells and so on. Here, microspheres were covalently integrated into 3D-printed frameworks to form composite scaffolds for bone repair. The frameworks composed of double bond modified gelatin (Gel-MA) and nano-hydroxyapatite (nHAP) provided strong support for related cells climbing and growth. Microspheres, which were made of Gel-MA and chondroitin sulfate A (CSA), were able to connect the frameworks like bridges, providing channels for cells migration. Additionally, CSA released from microspheres promoted the migration of osteoblasts and enhanced osteogenesis. The composite scaffolds could effectively repair mouse skull defect and improve MC3T3-E1 osteogenic differentiation. These observations confirm the bridging effect of microspheres rich in chondroitin sulfate and also determine that the composite scaffold can be as a promising candidate for enhanced bone repair.
大多数骨修复支架为多连通通道结构，但这种中空结构不利于活性因子、细胞等的传递。这里，将微球共价整合到 3D 打印的支架中，形成用于骨修复的复合支架。由双健改性明胶（Gel-MA）和纳米羟基磷灰石（nHAP）组成的支架为相关细胞的爬行和生长提供了强有力的支持。由 Gel-MA 和硫酸软骨素 A（CSA）制成的微球像桥一样连接支架，为细胞迁移提供了通道。此外，从微球中释放的 CSA 促进成骨细胞的迁移，增强成骨作用。复合支架可有效修复小鼠颅骨缺损，促进 MC3T3-E1 成骨分化。这些观察结果证实了富含硫酸软骨素的微球的桥接作用，并确定了复合支架可作为增强骨修复的有前途的候选物。
