Department of Pediatric Dentistry & Community Oral Health, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.
Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.
Methods Mol Biol. 2024;2835:325-337. doi: 10.1007/978-1-0716-3995-5_28.
Gelatin, a protein derivative from collagen, is a versatile material with promising applications in tissue engineering. Among the various forms of gelatin scaffolds, nanofibrous gelatin microspheres (NFGMs) are attracting research efforts due to their fibrous nature and injectability. However, current methods for synthesizing nanofibrous gelatin microspheres (NFGMs) have limitations, such as wide size distributions and the use of toxic solvents. To address these challenges, the article introduces a novel approach. First, it describes the creation of a microfluidic device using readily available supplies. Subsequently, it outlines a unique process for producing monodispersed NFGMs through a combination of the microfluidic device and thermally induced phase separation (TIPS). This innovative method eliminates the need for sieving and the use of toxic solvents, making it a more ecofriendly and efficient alternative.
明胶是一种源自胶原蛋白的蛋白质衍生物，是一种用途广泛的材料，在组织工程中有广阔的应用前景。在各种形式的明胶支架中，纳米纤维明胶微球（NFGMs）因其纤维状和可注射性而引起了研究人员的关注。然而，目前合成纳米纤维明胶微球（NFGMs）的方法存在局限性，例如尺寸分布较宽以及使用有毒溶剂。为了解决这些挑战，本文介绍了一种新方法。首先，它描述了使用现成的材料创建微流控设备的过程。随后，它概述了一种通过微流控设备和热诱导相分离（TIPS）相结合来生产单分散 NFGMs 的独特工艺。这种创新方法消除了筛分和使用有毒溶剂的需要，使其成为一种更环保、更高效的替代方法。
