College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China; Med-X Center for Materials, Sichuan University, Chengdu 610065, China.
Biomater Adv. 2022 Jul;138:212873. doi: 10.1016/j.bioadv.2022.212873. Epub 2022 May 19.
Developing non-antibiotic-dependent antibacterial hydrogels for repairing infected skin defect remains largely unexplored. Herein, a synergic photothermal and synergic antibacterial hydrogel was designed via one-pot approach to cope with full-thickness skin wound infection. In this work, the mixture of silver‑sodium lignin sulfonate nanoparticles (Ag-SLS NPs) and polypyrrole-polydopamine nanoparticles (PPy-PDA NPs) for the first time as synergic photothermal agent was introduced into poly (ethylene glycol) diacrylate (PEGDA) to form Ag-SLS/PPy-PDA@PEGDA hydrogel. Thanks to the near-infrared light into heat conversion capacity of both Ag and PPy-PDA NPs, the formed hydrogel shows ultrahigh photothermal activity, and outstanding antibacterial activity to both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria in vitro. Additionally, in vivo assay indicates that the hydrogel has outstanding long-term antibacterial effect due to the photothermal sterilization along with Ag ions release. Overall, the dual-synergic photothermal and antibacterial effects of the Ag-SLS/PPy-PDA@PEGDA hydrogel could highly accelerate infection wound healing, and thus has great potential to be used for tissue engineering.
开发不依赖抗生素的抗菌水凝胶来修复感染性皮肤缺损在很大程度上仍未得到探索。在此，通过一锅法设计了协同光热和协同抗菌水凝胶，以应对全层皮肤伤口感染。在这项工作中，首次将银-磺酸钠木质素纳米粒子（Ag-SLS NPs）和聚吡咯-聚多巴胺纳米粒子（PPy-PDA NPs）的混合物作为协同光热剂引入聚乙二醇二丙烯酸酯（PEGDA）中，形成 Ag-SLS/PPy-PDA@PEGDA 水凝胶。由于 Ag 和 PPy-PDA NPs 的近红外光转化为热的能力，所形成的水凝胶表现出超高的光热活性，对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌均具有出色的体外抗菌活性。此外，体内试验表明，由于光热杀菌和 Ag 离子释放，水凝胶具有出色的长期抗菌效果。总的来说，Ag-SLS/PPy-PDA@PEGDA 水凝胶的双重协同光热和抗菌作用可以高度促进感染伤口愈合，因此具有很大的潜力用于组织工程。
