Shinoda Kenta, Suganami Akiko, Moriya Yasumitsu, Yamashita Masamichi, Tanaka Tsutomu, Suzuki Akane S, Suito Hiroshi, Akutsu Yasunori, Saito Kengo, Shinozaki Yoko, Isojima Kazuoki, Nakamura Naohito, Miyauchi Yasushi, Shirasawa Hiroshi, Matsubara Hisahiro, Okamoto Yoshiharu, Nakayama Toshinori, Tamura Yutaka
Department of Immunology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Department of Bioinformatics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Molecular Chirality Research Center, Chiba University, Chiba 263-8522, Japan.
Photodiagnosis Photodyn Ther. 2022 Sep;39:103041. doi: 10.1016/j.pdpdt.2022.103041. Epub 2022 Jul 29.
Phototheranostics represents a highly promising paradigm for cancer therapy, although selecting an appropriate optical imager and sensitizer for clinical use remains challenging.
Liposomally formulated phospholipid-conjugated indocyanine green, denoted as LP-iDOPE, was developed as phototheranostic nanoparticle and its cancer imaging-mediated photodynamic reaction, defined as the immune response induced by photodynamic and photothermal effects, was evaluated with a near-infrared (NIR)-light emitting diode (LED) light irradiator.
Using in vivo NIR fluorescence imaging, we demonstrated that LP-iDOPE was selectively delivered to tumor sites with high accumulation and a long half-life. Following low-intensity NIR-LED light irradiation on the tumor region of LP-iDOPE accumulated, effector CD8 T cells were activated at the secondary lymphoid organs, migrated, and subsequently released cytokines including interferon-γ and tumor necrosis factor-α, resulting in effective tumor regression.
Our anti-cancer strategy based on tumor-specific LP-iDOPE accumulation and low-intensity NIR-LED light irradiation to the tumor regions, i.e., photodynamic reaction, represents a promising approach to noninvasive cancer therapy.
光诊疗学是一种极具前景的癌症治疗模式，不过为临床应用选择合适的光学成像剂和敏化剂仍具有挑战性。
开发了脂质体包裹的磷脂共轭吲哚菁绿，即LP-iDOPE，作为光诊疗纳米颗粒，并使用近红外（NIR）发光二极管（LED）光照射器评估其癌症成像介导的光动力反应，该反应定义为由光动力和光热效应诱导的免疫反应。
通过体内近红外荧光成像，我们证明LP-iDOPE能够以高蓄积和长半衰期选择性地递送至肿瘤部位。在对蓄积有LP-iDOPE的肿瘤区域进行低强度近红外LED光照射后，效应性CD8 T细胞在二级淋巴器官被激活，迁移并随后释放包括干扰素-γ和肿瘤坏死因子-α在内的细胞因子，从而实现有效的肿瘤消退。
我们基于肿瘤特异性LP-iDOPE蓄积和对肿瘤区域进行低强度近红外LED光照射的抗癌策略，即光动力反应，是一种有前景的非侵入性癌症治疗方法。
