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开发微卫星标记物用于深海珊瑚 Madracis myriaster(Pocilloporidae:珊瑚纲)。

Development of microsatellites markers for the deep coral Madracis myriaster (Pocilloporidae: Anthozoa).

机构信息

Faculty of Science and Engineering, Manchester Metropolitan University-MMU, Manchester, UK.

Instituto de Investigaciones Marinas y Costeras de Colombia-INVEMAR, Santa Marta D.T.C.H., Santa Marta, Colombia.

出版信息

Sci Rep. 2022 Aug 1;12(1):13193. doi: 10.1038/s41598-022-14322-7.

Abstract

In 2013 Colombia made an important step towards the construction and management of Marine Protected Areas (MPAs) by establishing the first Deep Corals National Park (PNNCP). Inside this MPA, the coral Madracis myriaster (Cnidaria: Pocilloporidae) was found as the main reef builder, offering habitat for many species of fish and invertebrates. In order to improve the study of deep-sea coral habitats, their connectivity and prospective management, nine new genetic markers (microsatellites) were developed for M. myriaster and tested in samples from PNNCP. We present the assessment of these markers, with a specificity for the deep coral, and its prospective use in future analysis for the PNNCP and other areas in the Caribbean and the Atlantic, where M. myriaster is reported. We also include an additional taxonomic analysis performed on samples of M. myriaster using scanning electron microscopy.

摘要

2013 年,哥伦比亚通过建立第一个深海珊瑚国家公园(PNNCP),在海洋保护区(MPA)的建设和管理方面迈出了重要一步。在这个 MPA 内,发现了主要的珊瑚礁建造者 Madracis myriaster(刺胞动物门:Pocilloporidae 科),为许多鱼类和无脊椎动物提供了栖息地。为了改善深海珊瑚栖息地、连通性和未来管理的研究,我们为 M. myriaster 开发了九个新的遗传标记(微卫星),并在 PNNCP 的样本中进行了测试。我们介绍了这些标记的评估结果,这些标记对深海珊瑚具有特异性,并有望在未来对 PNNCP 及加勒比海和大西洋其他地区的分析中使用,因为这些地区都有 M. myriaster 的报告。我们还包括对使用扫描电子显微镜采集的 M. myriaster 样本进行的额外分类学分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cfe/9343366/3000607127d9/41598_2022_14322_Fig1_HTML.jpg

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