School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, P. R. China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, P. R. China.
Nat Prod Res. 2023 May;37(10):1714-1718. doi: 10.1080/14786419.2022.2104275. Epub 2022 Aug 1.
An analysis method was developed for the separation and quantification of hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin by using HPLC-UV. Single factor experiments and Box-Behnken Designs were used to optimize separation of four flavonoids, in which a gradient elution method was adopted with 99% acetonitrile and 0.1% formic acid as mobile phases at a flow rate of 0.9 mL/min. A C column was used with a column temperature of 35 °C. LODs and LOQs were below 0.84 µg/mL and 2.84 µg/mL, respectively. Linearity with good correlation coefficients ( > 0.99, = 5) was attained, recovery rate of four flavonoids ranged from 88% to 130%, the RSD indicating results precision for analyzing hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin ranged from 1.2% to 4.6%. Finally, the present method could be successfully applied to identify and quantify hesperidin, neohesperidin and hesperetin in and .
建立了一种采用高效液相色谱-紫外检测法（HPLC-UV）分离和定量分析橙皮苷、新橙皮苷、新橙皮苷二氢查尔酮和柚皮素的方法。通过单因素实验和 Box-Behnken 设计对四种黄酮类化合物的分离条件进行优化，采用梯度洗脱法，流动相为 99%乙腈和 0.1%甲酸，流速为 0.9mL/min。采用 C 柱，柱温为 35℃。LOD 和 LOQ 分别低于 0.84µg/mL 和 2.84µg/mL。线性关系良好，相关系数（r）均大于 0.99（n=5）。四种黄酮类化合物的回收率在 88%至 130%之间，分析橙皮苷、新橙皮苷、新橙皮苷二氢查尔酮和柚皮素的 RSD 表明结果精密度在 1.2%至 4.6%之间。最后，该方法可成功应用于 和 中橙皮苷、新橙皮苷和柚皮素的鉴别和定量分析。
