Baranowska Irena, Magiera Sylwia
Silesian University of Technology, Faculty of Chemistry, Department of Analytical Chemistry, 7 M. Strzody St, 44-100 Gliwice, Poland.
J AOAC Int. 2011 May-Jun;94(3):786-94.
A simple and fast ultra-high-performance liquid chromatography (UHPLC) method was developed for the identification and quantification of the following flavonoids in red wine: (+/-)-catechin, (-)-epicatechin, rutin, quercitrin, hesperidin, neohesperidin, (+/-)-naringenin, hesperetin, and chrysin. Chromatographic separation of the flavonoids was performed on a Chromolith Fast Gradient C18e column. A gradient elution was used with mobile phases consisting of 0.1% formic acid in water and acetonitrile. UV detection was performed at 280 nm. A complete separation of flavonoids was possible within 6 min. The calibration curves showed good linearity (R2 > or = 0.9990) in the selected range of each analyte; the LOD ranged between 0.06 and 0.19 microg/mL. An optimized sample preparation method utilized SPE. The Oasis HLB column with the highest recoveries was selected for the preconcentration step. This method was successfully applied to the determination of these flavonoids in the red wine samples with excellent results.
建立了一种简单快速的超高效液相色谱（UHPLC）方法，用于鉴定和定量红酒中的以下黄酮类化合物：（±）-儿茶素、（-）-表儿茶素、芦丁、槲皮苷、橙皮苷、新橙皮苷、（±）-柚皮素、橙皮素和白杨素。黄酮类化合物的色谱分离在Chromolith Fast Gradient C18e柱上进行。采用梯度洗脱，流动相由0.1%甲酸水溶液和乙腈组成。在280nm处进行紫外检测。6分钟内即可实现黄酮类化合物的完全分离。校准曲线在各分析物的选定范围内显示出良好的线性（R2≥0.9990）；检测限在0.06至0.19μg/mL之间。优化的样品制备方法采用固相萃取（SPE）。选择回收率最高的Oasis HLB柱进行预浓缩步骤。该方法成功应用于红酒样品中这些黄酮类化合物的测定，结果良好。
