Central Laboratory, the Second Hospital of Tianjin Medical University, Tianjin 300211, China.
Central Laboratory, the Second Hospital of Tianjin Medical University, Tianjin 300211, China.
Colloids Surf B Biointerfaces. 2022 Oct;218:112719. doi: 10.1016/j.colsurfb.2022.112719. Epub 2022 Jul 27.
Polyetheretherketone (PEEK) has been widely applied for orthopedic and oral implants due to its excellent mechanical properties, biocompatibility, and radiolucency. However, its bioinert and the lack of anti-microbial activity limit its application. We modified the PEEK surface with Ta/Cu co-implantation using plasma immersion ion-implantation technology. After implantation of Ta/Cu ions, the morphology and roughness of the PEEK surface were not significantly changed at micron level. We estimated the cytocompatibility, anti-microbial ability, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs) of the modified surfaces in vitro. Compared to the untreated surfaces, the Ta ion-treated surface showed improved adhesion, proliferation, ALP activity, ECM mineralization, and osteogenic gene expression of BMSCs. Further, the Cu ion-treated surface showed reduced initial adhesion and proliferation of Escherichia coli and Staphylococcus aureus in vitro and proliferation of Staphylococcus aureus in the mouse subcutaneous implant-associated infection model. According to a rat bone repair model, all Ta ion-implanted groups demonstrated improved new bone formation. In summary, Ta/Cu ion co-impanation improved anti-microbial activity and promoted osseointegration of the PEEK surface.
聚醚醚酮（PEEK）因其优异的机械性能、生物相容性和放射可透性而被广泛应用于骨科和口腔植入物。然而，其生物惰性和缺乏抗菌活性限制了其应用。我们使用等离子体浸没离子注入技术对 PEEK 表面进行 Ta/Cu 共植入改性。植入 Ta/Cu 离子后，PEEK 表面的形貌和粗糙度在微米级水平上没有明显变化。我们评估了体外改性表面对大鼠骨髓间充质干细胞（BMSCs）的细胞相容性、抗菌能力和成骨分化。与未处理表面相比，Ta 离子处理表面显示出 BMSCs 更好的黏附、增殖、ALP 活性、细胞外基质矿化和成骨基因表达。此外，Cu 离子处理表面在体外显示出对大肠杆菌和金黄色葡萄球菌初始黏附、增殖的减少，以及在小鼠皮下植入相关感染模型中对金黄色葡萄球菌增殖的减少。根据大鼠骨修复模型，所有 Ta 离子植入组均表现出改善的新骨形成。综上所述，Ta/Cu 离子共注入提高了 PEEK 表面的抗菌活性并促进了其骨整合。
