School of Environment and Energy, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Key Lab Pollution Control & Ecosystem Restoration in Industry Cluster, Ministry of Education, Guangzhou, 510006, Guangdong, China.
Environ Sci Pollut Res Int. 2022 Sep;29(42):64244-64251. doi: 10.1007/s11356-022-22260-0. Epub 2022 Aug 3.
Arylsulfatase and β-glucuronidase are the two substantial enzymes having a significant role in the cleavage of conjugated natural estrogens (C-NEs). The present study reports that arylsulfatase and β-glucuronidase have been abundantly found in the digestive tracts of Cipangopaludina chinensis; in which, their corresponding activities were 60 and 5 U/g wet waste, respectively. The arylsulfatase from Cipangopaludina chinensis could show high activity at low temperatures. Hence, its activity still remained at 53.2% of maximal activity even at an extremely low temperature of 4 ℃; while the corresponding activities of arylsulfatase from Helix pomatia or activated sludge were less than 20% and 10%, respectively. The arylsulfatase and β-glucuronidase from Cipangopaludina chinensis could efficiently cleave C-NEs suggesting that they could be alternative enzymes derived from Helix pomatia that are used for cleavage of conjugated compounds in environmental or biological sample analysis. Meanwhile, they might also be used to enhance the cleavage of C-NEs in municipal wastewater.
芳基硫酸酯酶和β-葡萄糖醛酸酶是两种重要的酶，在共轭天然雌激素（C-NEs）的裂解中发挥重要作用。本研究报告称，芳基硫酸酯酶和β-葡萄糖醛酸酶在中华圆田螺的消化道中大量存在；其中，它们的相应活性分别为 60 和 5 U/g 湿废物。中华圆田螺的芳基硫酸酯酶在低温下表现出高活性。因此，即使在极低的温度 4℃下，其活性仍保持在最大活性的 53.2%；而来自大蜗牛或活性污泥的芳基硫酸酯酶的相应活性分别小于 20%和 10%。中华圆田螺的芳基硫酸酯酶和β-葡萄糖醛酸酶能够有效地裂解 C-NEs，这表明它们可以替代大蜗牛中用于环境或生物样品分析中裂解共轭化合物的酶。同时，它们也可能用于增强城市废水中 C-NEs 的裂解。
