Liu Gongzhen, Zhong Kangyu, Gong Shanmin, Li Xinru, Li Yanshen
College of Agriculture and Forestry, Linyi University, Linyi, China.
Department of Marine Product Quality and Safety Inspection Key Laboratory, Yantai University, Yantai, China.
Front Chem. 2024 Aug 29;12:1416942. doi: 10.3389/fchem.2024.1416942. eCollection 2024.
is a common freshwater mollusk that is widely distributed worldwide, especially in China. In our research, 1,382 proteins and 1,039 phosphorylated proteins were identified from carcasses, and 690 differentially expressed proteins (DEPs) were quantified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the DEPs are involved in cellular processes, single-organism processes, metabolic processes, developmental processes, localization, and biological regulation. The phosphorylated proteins were found to be related to the Rap1 signaling pathway, Ras signaling pathway, calcium signaling pathway, and longevity-regulating pathways. Moreover, we also identified important regulatory enzymes, such as guanylate cyclase, tyrosine protein kinase, receptor protein tyrosine kinase, and glyoxylate reductase/hydroxypyruvate reductase. Notably, we found guanylate cyclase to be present in multiple signaling pathways, including the Rap1 signaling pathway, calcium signaling pathway, Ras signaling pathway, insulin secretion, longevity regulating pathway, glutamatergic synapse, circadian entrainment, and gap junction. This enzyme may play a crucial role in regulating molecular mechanisms in In summary, proteomic and phosphoproteomic analyses of carcasses displayed significant differences among different geographical isolates, which helps enhance our understanding of food nutrition, signaling pathways, and metabolic mechanisms in .
是一种常见的淡水软体动物，在全球广泛分布，尤其是在中国。在我们的研究中，从其尸体中鉴定出1382种蛋白质和1039种磷酸化蛋白质，并对690种差异表达蛋白质（DEPs）进行了定量分析。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，这些差异表达蛋白质参与细胞过程、单细胞过程、代谢过程、发育过程、定位和生物调节。发现磷酸化蛋白质与Rap1信号通路、Ras信号通路、钙信号通路和寿命调节通路有关。此外，我们还鉴定出了重要的调节酶，如鸟苷酸环化酶、酪氨酸蛋白激酶、受体蛋白酪氨酸激酶和乙醛酸还原酶/羟基丙酮酸还原酶。值得注意的是，我们发现鸟苷酸环化酶存在于多个信号通路中，包括Rap1信号通路、钙信号通路、Ras信号通路、胰岛素分泌、寿命调节通路、谷氨酸能突触、昼夜节律夹带和缝隙连接。这种酶可能在调节[未提及的生物名称]的分子机制中起关键作用。总之，对[未提及的生物名称]尸体的蛋白质组学和磷酸蛋白质组学分析显示不同地理分离株之间存在显著差异，这有助于增强我们对[未提及的生物名称]的食物营养、信号通路和代谢机制的理解。
