Department of Physiology, King George's Medical University, Lucknow, UP, India.
Speciality of Pathology and Cancer Genetics, Kalyan Singh Super Speciality Cancer Institute, Lucknow, UP, India.
Clin Transl Oncol. 2022 Nov;24(11):2191-2199. doi: 10.1007/s12094-022-02879-8. Epub 2022 Aug 2.
Beta 2-Adrenergic Receptor (β2-AR) is significantly overexpressed in various types of malignancies, which is associated with the worst prognosis. However, the role of β2-AR in oral cancer is not well identified. The present study aimed at investigating the β2-AR gene expression and its significance in relation with the clinicopathological features and overall survival of oral squamous cell carcinoma (OSCC) patients.
Immunohistochemistry, western blot and quantitative real-time PCR techniques were used to analyze β2-AR protein and mRNA levels in a total of 65 histopathologically confirmed OSCC tissues (case group) and 65 normal tissues (control group) from the oral cavity.
Out of the total of 65 OSCC tissues, 41 tissues (63.1%) exhibited high expression for β2-AR protein. Percent positivity and relative density (mean ± SD) of protein were higher in the case group as compared to the control group (positivity 40.31 ± 3.01 vs. 20.46 ± 1.93, p < 0.001; density 2.77 ± 1.17 vs. 1.28 ± 0.37, p < 0.001). In addition, β2-AR mRNA level was also upregulated in patients compared to the controls (2.36 ± 1.30 vs. 1.09 ± 0.42, p < 0.001) and showed a positive correlation with immunostaining of protein in OSCC (r = 0.48, p = 0.011). High β2-AR protein expression was significantly associated with multiple risk habits (p = 0.045), histological differentiation (p = 0.013), clinical TNM stages (p = 0.014), and poor survival (p = 0.006) of patients. In the Cox proportional hazards model, β2-AR was identified as a prognostic biomarker of OSCC (p = 0.047).
β2-AR protein level is identified as an independent significant prognostic factor in patients with oral carcinoma.
β2-肾上腺素能受体（β2-AR）在各种类型的恶性肿瘤中显著过表达，与预后最差相关。然而，β2-AR 在口腔癌中的作用尚未明确。本研究旨在探讨β2-AR 基因表达及其与口腔鳞状细胞癌（OSCC）患者临床病理特征和总生存的关系。
采用免疫组织化学、Western blot 和实时定量 PCR 技术分析了 65 例经组织病理学证实的口腔 OSCC 组织（病例组）和 65 例口腔正常组织（对照组）中β2-AR 蛋白和 mRNA 水平。
在 65 例 OSCC 组织中，有 41 例（63.1%）β2-AR 蛋白表达高。病例组的阳性率和相对密度（平均值±标准差）均高于对照组（阳性率 40.31±3.01%对 20.46±1.93%，p<0.001；密度 2.77±1.17 对 1.28±0.37，p<0.001）。此外，与对照组相比，患者的β2-AR mRNA 水平也升高（2.36±1.30 对 1.09±0.42，p<0.001），并与 OSCC 中蛋白免疫染色呈正相关（r=0.48，p=0.011）。高β2-AR 蛋白表达与多种危险因素（p=0.045）、组织学分化（p=0.013）、临床 TNM 分期（p=0.014）和不良预后（p=0.006）显著相关。在 Cox 比例风险模型中，β2-AR 被确定为 OSCC 的预后生物标志物（p=0.047）。
β2-AR 蛋白水平被确定为口腔癌患者独立的显著预后因素。
