Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China.
College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China.
J Vet Sci. 2022 Jul;23(4):e57. doi: 10.4142/jvs.22042.
Classical swine fever virus (CSFV), the causative agent of classical swine fever (CFS), is a highly contagious disease that poses a serious threat to Chinese pig populations.
Many provinces of China, such as Shandong, Henan, Hebei, Heilongjiang, and Liaoning provinces, have reported epidemics of CSFV, while the references to the epidemic of CSFV in Yunnan province are rare. This study examined the epidemic characteristics of the CSFV in Yunnan province.
In this study, 326 tissue samples were collected from different regions in Yunnan province from 2015 to 2021. A reverse transcription-polymerase chain reaction (RT-PCR), sequences analysis, and phylogenetic analysis were performed for the pathogenic detection and analysis of these 326 clinical specimens.
Approximately 3.37% (11/326) of specimens tested positive for the CSFV by RT-PCR, which is lower than that of other regions of China. Sequence analysis of the partial E2 sequences of eleven CSFV strains showed that they shared 89.0-100.0% nucleotide (nt) and 95.0-100.0% amino acid (aa) homology, respectively. Phylogenetic analysis showed that these novel isolates belonged to the subgenotypes 2.1c and 2.1d, with subgenotype 2.1c being predominant.
The CSFV was sporadic in China's Yunnan province from 2015 to 2021. Both 2.1c and 2.1d subgenotypes were found in this region, but 2.1c was dominant.
经典猪瘟病毒（CSFV）是引起经典猪瘟（CSF）的病原体，是一种高度传染性疾病，对中国猪群构成严重威胁。
中国许多省份，如山东、河南、河北、黑龙江和辽宁等，都报告了 CSFV 的流行，但云南省 CSFV 流行的报道很少。本研究检测了云南省 CSFV 的流行特征。
本研究从 2015 年至 2021 年采集了云南省不同地区的 326 份组织样本。对这些 326 份临床样本进行了逆转录-聚合酶链反应（RT-PCR）、序列分析和系统进化分析，以进行病原检测和分析。
通过 RT-PCR 检测，约 3.37%（11/326）的标本呈 CSFV 阳性，低于中国其他地区。对 11 株 CSFV 株的部分 E2 序列的分析表明，它们的核苷酸（nt）和氨基酸（aa）同源性分别为 89.0-100.0%和 95.0-100.0%。系统进化分析表明，这些新分离株属于 2.1c 和 2.1d 亚群，其中 2.1c 亚群占优势。
2015 年至 2021 年，CSFV 在云南省呈散发状态。该地区同时存在 2.1c 和 2.1d 亚群，但 2.1c 亚群占优势。
