Zhu Zheng, Wang Tianxingzi, Lan Jinping, Ma Jinjiao, Xu Haiqing, Yang Zexi, Guo Yalu, Chen Yue, Zhang Jianshuo, Dou Shijuan, Yang Ming, Li Liyun, Liu Guozhen
College of Life Sciences, Hebei Agricultural University, 2596 Lekai South Street, West Campus, Baoding, 071001, Hebei, China.
Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, 071001, China.
Rice (N Y). 2022 Aug 3;15(1):41. doi: 10.1186/s12284-022-00590-4.
Rice bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases affecting rice production worldwide. Xa21 was the first disease resistance gene cloned in rice, which encodes a receptor kinase and confers broad resistance against Xoo stains. Dozens of components in the Xa21-mediated pathway have been identified in the past decades, however, the involvement of mitogen-activated protein kinase (MAPK) genes in the pathway has not been well described. To identify MAPK involved in Xa21-mediated resistance, the level of MAPK proteins was profiled using Western blot analysis. The abundance of OsMPK17 (MPK17) was found decreased during the rice-Xoo interaction in the background of Xa21. To investigate the function of MPK17, MPK17-RNAi and over-expression (OX) transgenic lines were generated. The RNAi lines showed an enhanced resistance, while OX lines had impaired resistance against Xoo, indicating that MPK17 plays negative role in Xa21-mediated resistance. Furthermore, the abundance of transcription factor WRKY62 and pathogenesis-related proteins PR1A were changed in the MPK17 transgenic lines when inoculated with Xoo. We also observed that the MPK17-RNAi and -OX rice plants showed altered agronomic traits, indicating that MPK17 also plays roles in the growth and development. On the basis of the current study and published results, we propose a "Xa21-MPK17-WRKY62-PR1A" signaling that functions in the Xa21-mediated disease resistance pathway. The identification of MPK17 advances our understanding of the mechanism underlying Xa21-mediated immunity, specifically in the mid- and late-stages.
水稻白叶枯病由稻黄单胞菌水稻致病变种（Xoo）引起，是影响全球水稻生产的最严重病害之一。Xa21是水稻中克隆的第一个抗病基因，它编码一种受体激酶，对多种Xoo菌株具有广谱抗性。在过去几十年中，已经鉴定出Xa21介导途径中的数十种组分，然而，丝裂原活化蛋白激酶（MAPK）基因在该途径中的作用尚未得到充分描述。为了鉴定参与Xa21介导抗性的MAPK，使用蛋白质免疫印迹分析来分析MAPK蛋白的水平。发现在Xa21背景下的水稻-Xoo相互作用过程中，OsMPK17（MPK17）的丰度降低。为了研究MPK17的功能，构建了MPK17-RNA干扰和过表达（OX）转基因系。RNA干扰系表现出增强的抗性，而过表达系对Xoo的抗性受损，表明MPK17在Xa21介导的抗性中起负作用。此外，接种Xoo后，MPK17转基因系中转录因子WRKY62和病程相关蛋白PR1A的丰度发生了变化。我们还观察到MPK17-RNA干扰和过表达水稻植株的农艺性状发生了改变，表明MPK17也在生长发育中起作用。基于目前的研究和已发表的结果，我们提出了一个在Xa21介导的抗病途径中起作用的“Xa21-MPK17-WRKY62-PR1A”信号通路。MPK17 的鉴定加深了我们对Xa21介导的免疫机制的理解，特别是在中期和后期。
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