利用时间进程转录组分析理解水稻穗瘟抗性和感病品种中的互作。
Understanding Rice- Interaction in Resistant and Susceptible Cultivars of Rice under Panicle Blast Infection Using a Time-Course Transcriptome Analysis.
机构信息
ICAR-National Institute for Plant Biotechnology, New Delhi 110012, India.
Jamia Hamdard, Hamdard Nagar, New Delhi 110012, India.
出版信息
Genes (Basel). 2021 Feb 20;12(2):301. doi: 10.3390/genes12020301.
Rice blast is a global threat to food security with up to 50% yield losses. Panicle blast is a more severe form of rice blast and the response of rice plant to leaf and panicle blast is distinct in different genotypes. To understand the specific response of rice in panicle blast, transcriptome analysis of blast resistant cultivar Tetep, and susceptible cultivar HP2216 was carried out using RNA-Seq approach after 48, 72 and 96 h of infection with along with mock inoculation. Transcriptome data analysis of infected panicle tissues revealed that 3553 genes differentially expressed in HP2216 and 2491 genes in Tetep, which must be the responsible factor behind the differential disease response. The defense responsive genes are involved mainly in defense pathways namely, hormonal regulation, synthesis of reactive oxygen species, secondary metabolites and cell wall modification. The common differentially expressed genes in both the cultivars were defense responsive transcription factors, NBS-LRR genes, kinases, pathogenesis related genes and peroxidases. In Tetep, cell wall strengthening pathway represented by PMR5, dirigent, tubulin, cell wall proteins, chitinases, and proteases was found to be specifically enriched. Additionally, many novel genes having DOMON, VWF, and PCaP1 domains which are specific to cell membrane were highly expressed only in Tetep post infection, suggesting their role in panicle blast resistance. Thus, our study shows that panicle blast resistance is a complex phenomenon contributed by early defense response through ROS production and detoxification, MAPK and LRR signaling, accumulation of antimicrobial compounds and secondary metabolites, and cell wall strengthening to prevent the entry and spread of the fungi. The present investigation provided valuable candidate genes that can unravel the mechanisms of panicle blast resistance and help in the rice blast breeding program.
稻瘟病是全球粮食安全的一大威胁，可导致高达 50%的产量损失。穗瘟是稻瘟病的一种更为严重的形式，不同基因型的水稻对叶片和穗部稻瘟病的反应明显不同。为了了解水稻在穗瘟中的特定反应，我们采用 RNA-Seq 方法，对具有抗穗瘟特性的 Tetep 品种和易感的 HP2216 品种进行了研究，在接种 后 48、72 和 96 小时以及模拟接种时，对叶片和穗部组织进行了转录组分析。对感染穗部组织的转录组数据分析表明，在 HP2216 中有 3553 个基因差异表达，在 Tetep 中有 2491 个基因差异表达，这些基因差异表达可能是导致不同疾病反应的原因。防御相关基因主要参与防御途径，如激素调节、活性氧物质的合成、次生代谢物和细胞壁修饰。在两个品种中共同差异表达的基因是防御相关的转录因子、NBS-LRR 基因、激酶、病程相关基因和过氧化物酶。在 Tetep 中，细胞壁强化途径（由 PMR5、导向蛋白、微管蛋白、细胞壁蛋白、几丁质酶和蛋白酶组成）被发现是特异性富集的。此外，许多具有 DOMON、VWF 和 PCaP1 结构域的新基因，这些结构域特异性地存在于细胞膜上，仅在 Tetep 感染后高度表达，表明它们在穗瘟抗性中的作用。因此，我们的研究表明，穗瘟抗性是一种复杂的现象，通过 ROS 产生和解毒、MAPK 和 LRR 信号转导、积累抗菌化合物和次生代谢物以及细胞壁强化来防止真菌的入侵和扩散，从而在早期防御反应中发挥作用。本研究提供了有价值的候选基因，这些基因可以揭示穗瘟抗性的机制，并有助于水稻稻瘟病的育种计划。
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