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水稻基因LRR1的沉默会损害水稻Xa21转录本积累和XA21介导的免疫。

Silencing of the Rice Gene LRR1 Compromises Rice Xa21 Transcript Accumulation and XA21-Mediated Immunity.

作者信息

Caddell Daniel F, Park Chang-Jin, Thomas Nicholas C, Canlas Patrick E, Ronald Pamela C

机构信息

Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA.

Present Address: Department of Bioresources Engineering and PERI, Sejong University, Seoul, 05006, Republic of Korea.

出版信息

Rice (N Y). 2017 Dec;10(1):23. doi: 10.1186/s12284-017-0162-5. Epub 2017 May 22.

DOI:10.1186/s12284-017-0162-5
PMID:28534133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5440417/
Abstract

BACKGROUND

The rice immune receptor XA21 confers resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. We previously demonstrated that an auxilin-like protein, XA21 BINDING PROTEIN 21 (XB21), positively regulates resistance to Xoo.

RESULTS

To further investigate the function of XB21, we performed a yeast two-hybrid screen. We identified 22 unique XB21 interacting proteins, including LEUCINE-RICH REPEAT PROTEIN 1 (LRR1), which we selected for further analysis. Silencing of LRR1 in the XA21 genetic background (XA21-LRR1Ri) compromises resistance to Xoo compared with control XA21 plants. XA21-LRR1Ri plants have reduced Xa21 transcript levels and reduced expression of genes that serve as markers of XA21-mediated activation. Overexpression of LRR1 is insufficient to alter resistance to Xoo in rice lines lacking XA21.

CONCLUSIONS

Taken together, our results indicate that LRR1 is required for wild-type Xa21 transcript expression and XA21-mediated immunity.

摘要

背景

水稻免疫受体XA21赋予对水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，Xoo）的抗性，Xoo是细菌性条斑病的病原体。我们之前证明，一种类辅助蛋白，XA21结合蛋白21（XB21），正向调控对Xoo的抗性。

结果

为进一步研究XB21的功能，我们进行了酵母双杂交筛选。我们鉴定出22种独特的与XB21相互作用的蛋白，包括富含亮氨酸重复序列蛋白1（LRR1），我们选择其进行进一步分析。与对照XA21植株相比，在XA21遗传背景下沉默LRR1（XA21-LRR1Ri）会损害对Xoo的抗性。XA21-LRR1Ri植株中Xa21转录水平降低，且作为XA21介导激活标记的基因表达减少。在缺乏XA21的水稻品系中过表达LRR1不足以改变对Xoo的抗性。

结论

综上所述，我们的结果表明LRR1是野生型Xa21转录表达和XA21介导的免疫所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/3d89ec0414f7/12284_2017_162_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/289eec3732df/12284_2017_162_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/9d41178e1b9a/12284_2017_162_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/a996c4457a3d/12284_2017_162_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/c6e655911678/12284_2017_162_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/3d89ec0414f7/12284_2017_162_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/289eec3732df/12284_2017_162_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/9d41178e1b9a/12284_2017_162_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/a996c4457a3d/12284_2017_162_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/c6e655911678/12284_2017_162_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8944/5440417/3d89ec0414f7/12284_2017_162_Fig5_HTML.jpg

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