Rolfsen W, Schröder H, Tibell C, Tibell M
Allergy. 1987 Apr;42(3):177-81. doi: 10.1111/j.1398-9995.1987.tb02196.x.
Cat flea sensitivity is considered one of the most important skin diseases in cats and dogs. Cat fleas, however, are also a growing allergen problem for humans. Cat flea-specific IgE antibodies were studied in serum samples from 70 patients with suspected cat allergy, using RAST-based techniques and the nitrocellulose immunoblotting method. Results from RAST studies, using cat and cat flea as allergosorbents, showed that 46% of the patients were RAST positive against both cat and cat flea. 9% of the patients were RAST positive only against the cat flea. The nitrocellulose immunoblotting experiments were in agreement with the RAST results showing specific IgE to cat flea. The results indicate that some cat-allergic patients have specific IgE both towards cat and cat flea but also that some of the patients with suspected cat allergy might have specific IgE towards the cat flea and not the cat. RAST-inhibition and immunoblotting experiments also indicate that the allergen composition of cat flea extract differs from that of cat extract, even if common allergens have been detected, leading to cross-reactivity in some sera.
猫蚤敏感性被认为是猫和狗最重要的皮肤疾病之一。然而，猫蚤对人类来说也是一个日益严重的过敏原问题。使用基于放射变应原吸附试验（RAST）的技术和硝酸纤维素免疫印迹法，对70例疑似猫过敏患者的血清样本中的猫蚤特异性IgE抗体进行了研究。以猫和猫蚤作为变应原吸附剂的RAST研究结果表明，46%的患者对猫和猫蚤的RAST检测均呈阳性。9%的患者仅对猫蚤的RAST检测呈阳性。硝酸纤维素免疫印迹实验与RAST结果一致，显示出对猫蚤的特异性IgE。结果表明，一些对猫过敏的患者对猫和猫蚤都有特异性IgE，但也有一些疑似猫过敏的患者可能对猫蚤而非猫有特异性IgE。RAST抑制和免疫印迹实验还表明，即使检测到了共同的过敏原，猫蚤提取物的过敏原组成与猫提取物不同，这导致了一些血清中的交叉反应。
