• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

控制胶质母细胞瘤治疗疗效的信号通路

The Signaling Pathways Controlling the Efficacy of Glioblastoma Therapy.

作者信息

Vasileva N S, Ageenko A B, Richter V A, Kuligina E V

机构信息

Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090 Russia.

出版信息

Acta Naturae. 2022 Apr-Jun;14(2):62-70. doi: 10.32607/actanaturae.11623.

DOI:10.32607/actanaturae.11623
PMID:35923561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9307987/
Abstract

The resistance of glioblastoma to existing therapies puts limits on quality-of-life improvements and patient survival with a glioblastoma diagnosis. The development of new effective glioblastoma therapies is based on knowledge about the mechanisms governing tumor resistance to therapeutic agents. Virotherapy is one of the most actively developing approaches to the treatment of malignant neoplasms: glioblastoma in particular. Previously, we demonstrated that the recombinant vaccinia virus VV-GMCSF-Lact exhibits cytotoxic activity and antitumor efficacy against human glioblastoma. However, the studied glioblastoma cell cultures had different sensitivities to the oncotoxic effect of the virus. In this study, we investigated cancer stem cell (CSC) surface markers in glioblastoma cells with different sensitivities to VV-GMCSFLact using flow cytometry and we assessed the levels of proteins affecting viral entry into cells and virus infection efficiency by western blotting. We showed that cell cultures more sensitive to VV-GMCSF-Lact are characterized by a greater number of cells with CSC markers and a lower level of activated Akt kinase. Akt probably inhibits lactaptin-induced apoptosis in virus-resistant cells. Hence, we suggest that the sensitivity of glioblastoma cells to the oncotoxic effect of VV-GMCSF-Lact is determined by the nature and extent of the disturbances in cell death regulation in various cultures. Further investigation of the factors affecting glioblastoma resistance to virotherapy will test this hypothesis and identify targets for antitumor therapy, combined with VV-GMCSF-Lact.

摘要

胶质母细胞瘤对现有疗法的耐药性限制了胶质母细胞瘤患者生活质量的改善和生存期。新型有效胶质母细胞瘤疗法的开发基于对肿瘤对治疗药物耐药机制的了解。病毒疗法是治疗恶性肿瘤（尤其是胶质母细胞瘤）最积极发展的方法之一。此前，我们证明重组痘苗病毒VV-GMCSF-Lact对人胶质母细胞瘤具有细胞毒性活性和抗肿瘤功效。然而，所研究的胶质母细胞瘤细胞培养物对该病毒的致癌毒性作用具有不同的敏感性。在本研究中，我们使用流式细胞术研究了对VV-GMCSF-Lact具有不同敏感性的胶质母细胞瘤细胞中的癌症干细胞（CSC）表面标志物，并通过蛋白质印迹法评估了影响病毒进入细胞和病毒感染效率的蛋白质水平。我们发现，对VV-GMCSF-Lact更敏感的细胞培养物的特征是具有CSC标志物的细胞数量更多，且活化的Akt激酶水平较低。Akt可能抑制了病毒抗性细胞中乳清蛋白诱导的细胞凋亡。因此，我们认为胶质母细胞瘤细胞对VV-GMCSF-Lact致癌毒性作用的敏感性取决于不同培养物中细胞死亡调控紊乱的性质和程度。对影响胶质母细胞瘤对病毒疗法耐药性的因素进行进一步研究将验证这一假设，并确定与VV-GMCSF-Lact联合使用的抗肿瘤治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/00174ef4b1fb/AN20758251-14-02-062-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/dd5a275d0361/AN20758251-14-02-062-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/0c8a36504158/AN20758251-14-02-062-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/466d05b91f4f/AN20758251-14-02-062-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/84e6c358c8f0/AN20758251-14-02-062-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/6176db997c74/AN20758251-14-02-062-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/00174ef4b1fb/AN20758251-14-02-062-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/dd5a275d0361/AN20758251-14-02-062-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/0c8a36504158/AN20758251-14-02-062-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/466d05b91f4f/AN20758251-14-02-062-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/84e6c358c8f0/AN20758251-14-02-062-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/6176db997c74/AN20758251-14-02-062-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba16/9307987/00174ef4b1fb/AN20758251-14-02-062-g006.jpg

相似文献

1
The Signaling Pathways Controlling the Efficacy of Glioblastoma Therapy.控制胶质母细胞瘤治疗疗效的信号通路
Acta Naturae. 2022 Apr-Jun;14(2):62-70. doi: 10.32607/actanaturae.11623.
2
Engineering of double recombinant vaccinia virus with enhanced oncolytic potential for solid tumor virotherapy.具有增强溶瘤潜力的双重组痘苗病毒用于实体瘤病毒疗法的工程构建
Oncotarget. 2016 Nov 8;7(45):74171-74188. doi: 10.18632/oncotarget.12367.
3
Double Recombinant Vaccinia Virus: A Candidate Drug against Human Glioblastoma.双重组痘苗病毒：一种抗人胶质母细胞瘤的候选药物。
Life (Basel). 2021 Oct 14;11(10):1084. doi: 10.3390/life11101084.
4
The Recombinant Oncolytic Virus VV-GMCSF-Lact and Chemotherapy Drugs against Human Glioma.重组溶瘤病毒VV-GMCSF-Lact与化疗药物治疗人类胶质瘤
Int J Mol Sci. 2024 Apr 11;25(8):4244. doi: 10.3390/ijms25084244.
5
Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact.溶瘤病毒 VV-GMCSF-Lact 感染胶质瘤细胞后的转录组变化
Cells. 2023 Nov 12;12(22):2616. doi: 10.3390/cells12222616.
6
Recombinant Vaccinia Viruses Coding Transgenes of Apoptosis-Inducing Proteins Enhance Apoptosis But Not Immunogenicity of Infected Tumor Cells.编码诱导细胞凋亡蛋白的重组痘苗病毒增强了感染肿瘤细胞的凋亡，但不增强其免疫原性。
Biomed Res Int. 2017;2017:3620510. doi: 10.1155/2017/3620510. Epub 2017 Aug 30.
7
Characterizing Aptamer Interaction with the Oncolytic Virus VV-GMCSF-Lact.表征适体与溶瘤病毒 VV-GMCSF-Lact 的相互作用
Molecules. 2024 Feb 14;29(4):848. doi: 10.3390/molecules29040848.
8
Oncolytic Vaccinia Virus Gene Modification and Cytokine Expression Effects on Tumor Infection, Immune Response, and Killing.溶瘤痘苗病毒基因修饰及其细胞因子表达对肿瘤感染、免疫反应和杀伤的影响。
Mol Cancer Ther. 2021 Aug;20(8):1481-1494. doi: 10.1158/1535-7163.MCT-20-0863. Epub 2021 May 27.
9
Luteolin enhances the antitumor efficacy of oncolytic vaccinia virus that harbors IL-24 gene in liver cancer cells.木犀草素增强携带 IL-24 基因的溶瘤痘苗病毒在肝癌细胞中的抗肿瘤疗效。
J Clin Lab Anal. 2021 Mar;35(3):e23677. doi: 10.1002/jcla.23677. Epub 2020 Dec 3.
10
An engineered oncolytic vaccinia virus encoding a single-chain variable fragment against TIGIT induces effective antitumor immunity and synergizes with PD-1 or LAG-3 blockade.一种工程化的溶瘤痘苗病毒，编码针对 TIGIT 的单链可变片段，可诱导有效的抗肿瘤免疫，并与 PD-1 或 LAG-3 阻断协同作用。
J Immunother Cancer. 2021 Dec;9(12). doi: 10.1136/jitc-2021-002843.

引用本文的文献

1
Characterizing Aptamer Interaction with the Oncolytic Virus VV-GMCSF-Lact.表征适体与溶瘤病毒 VV-GMCSF-Lact 的相互作用
Molecules. 2024 Feb 14;29(4):848. doi: 10.3390/molecules29040848.

本文引用的文献

1
Double Recombinant Vaccinia Virus: A Candidate Drug against Human Glioblastoma.双重组痘苗病毒：一种抗人胶质母细胞瘤的候选药物。
Life (Basel). 2021 Oct 14;11(10):1084. doi: 10.3390/life11101084.
2
Cancer cell heterogeneity & plasticity in glioblastoma and brain tumors.胶质母细胞瘤和脑肿瘤中的癌细胞异质性与可塑性
Semin Cancer Biol. 2022 Jul;82:162-175. doi: 10.1016/j.semcancer.2021.02.014. Epub 2021 Feb 25.
3
Vaccinia Virus Activation and Antagonism of Cytosolic DNA Sensing.牛痘病毒激活和细胞溶质 DNA 感应拮抗作用。
Front Immunol. 2020 Oct 1;11:568412. doi: 10.3389/fimmu.2020.568412. eCollection 2020.
4
Advances and potential pitfalls of oncolytic viruses expressing immunomodulatory transgene therapy for malignant gliomas.表达免疫调节转基因治疗恶性脑胶质瘤的溶瘤病毒的进展和潜在陷阱。
Cell Death Dis. 2020 Jun 25;11(6):485. doi: 10.1038/s41419-020-2696-5.
5
Management of glioblastoma: State of the art and future directions.脑胶质瘤的治疗：现状与未来方向。
CA Cancer J Clin. 2020 Jul;70(4):299-312. doi: 10.3322/caac.21613. Epub 2020 Jun 1.
6
Understanding Glioblastoma Biomarkers: Knocking a Mountain with a Hammer.理解胶质母细胞瘤标志物：以卵击石。
Cells. 2020 May 16;9(5):1236. doi: 10.3390/cells9051236.
7
Glioblastoma Stem Cells: Driving Resilience through Chaos.胶质母细胞瘤干细胞：通过混沌驱动韧性。
Trends Cancer. 2020 Mar;6(3):223-235. doi: 10.1016/j.trecan.2020.01.009. Epub 2020 Feb 3.
8
Is there a causal link between PTEN deficient tumors and immunosuppressive tumor microenvironment?PTEN缺陷型肿瘤与免疫抑制性肿瘤微环境之间是否存在因果联系？
J Transl Med. 2020 Jan 30;18(1):45. doi: 10.1186/s12967-020-02219-w.
9
Feasibility study of using high-throughput drug sensitivity testing to target recurrent glioblastoma stem cells for individualized treatment.利用高通量药物敏感性测试靶向复发性胶质母细胞瘤干细胞进行个体化治疗的可行性研究。
Clin Transl Med. 2019 Dec 30;8(1):33. doi: 10.1186/s40169-019-0253-6.
10
Pivotal role of PDK1 in megakaryocyte cytoskeletal dynamics and polarization during platelet biogenesis.PDK1 在巨核细胞细胞骨架动力学和血小板发生过程中的极化中的关键作用。
Blood. 2019 Nov 21;134(21):1847-1858. doi: 10.1182/blood.2019000185.