Grant Richard, Kizu Joanne, Graham Melissa, McCallum Fiona, McPherson Brady, Auliff Alyson, Kaminiel Peter, Liu Wenjun
Arbovirology Department, Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, Brisbane, Queensland, Australia.
Queensland Institute of Medical Research-Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
IJID Reg. 2022 Jul 16;4:131-133. doi: 10.1016/j.ijregi.2022.07.006. eCollection 2022 Sep.
The Papua New Guinea (PNG) Health Department retrospectively reported six cases of Zika virus (ZIKV) from a cohort of febrile patients during outbreaks of dengue and malaria in 2016. However, the transmission of ZIKV remains unclear due to lack of testing capability. This study aimed to determine the level of immunity to ZIKV among PNG military personnel (PNGMP) in 2019.
Sera of 208 PNGMP recruited in April 2019 was tested for the presence of anti-ZIKV immunoglobulin G (IgG) and M (IgM) antibodies using Euroimmun IgG/IgM detection kits, and anti-ZIKV neutralizing antibody (Nab) against a ZIKV African strain on all anti-ZIKV-IgG/IgM samples.
Anti-ZIKV seropositivity of these sera was as follows: IgG, 67%; IgM, 9%; and Nab, 65%. Five of 19 anti-ZIKV-IgM samples had anti-ZIKV-Nab titres ≥20, as well as an anti-ZIKV-Nab titre ratio ≥4 compared with the Nab titres of four anti-dengue serotypes, so met the criteria of the World Health Organization (WHO) for confirmed ZIKV infection.
The prevalence of anti-ZIKV-Nab of 65% suggests that there are high levels of ZIKV exposure among PNGMP. Five of the 19 anti-ZIKV-IgM samples met the WHO criteria for confirmed ZIKV infection, suggesting a recent undetected outbreak in PNGMP. These results provide better understanding of the current ZIKV epidemic status in PNGMP.
巴布亚新几内亚（PNG）卫生部回顾性报告了2016年登革热和疟疾疫情期间一组发热患者中的6例寨卡病毒（ZIKV）病例。然而，由于缺乏检测能力，ZIKV的传播情况仍不清楚。本研究旨在确定2019年PNG军事人员（PNGMP）中对ZIKV的免疫水平。
使用欧蒙免疫球蛋白G（IgG）/免疫球蛋白M（IgM）检测试剂盒对2019年4月招募的208名PNGMP的血清进行抗ZIKV IgG和IgM抗体检测，并对所有抗ZIKV-IgG/IgM样本检测针对ZIKV非洲株的抗ZIKV中和抗体（Nab）。
这些血清的抗ZIKV血清阳性率如下：IgG为67%；IgM为9%；Nab为65%。19份抗ZIKV-IgM样本中有5份的抗ZIKV-Nab滴度≥20，并且与4种抗登革热血清型的Nab滴度相比，抗ZIKV-Nab滴度比值≥4，因此符合世界卫生组织（WHO）确诊ZIKV感染的标准。
抗ZIKV-Nab患病率为65%，表明PNGMP中ZIKV暴露水平较高。19份抗ZIKV-IgM样本中有5份符合WHO确诊ZIKV感染的标准，表明PNGMP近期存在未被发现的疫情。这些结果有助于更好地了解PNGMP中当前的ZIKV流行状况。
