Genotypic and Phenotypic Expression of Antibiotic Resistance Patterns of Uropathogenic Enterobacteriaceae.

AIM

To determine the antibiotic resistance patterns, detection of carbapenemase genes in uropathogenic bacilli belonging to the Enterobacteriaceae family and to correlate it with clinical data.

MATERIALS AND METHODS

Identification and antibiotic sensitivity testing of the uropathogenic Enterobacteriaceae was done by using VITEK2 Compact (C) system. Multiplex PCR was used to detect , and genes.

RESULTS

Out of 1602 urine samples, 417 (26%) showed significant growth, and in these 311 (74.6%) belonged to the family. showed a relatively low rate of resistance to nitrofurantoin (17/205; 8.3%), with the majority of the isolates showing a MIC value of ≤16 µg/mL when compared to spp. (55/86; 64%), with MIC values for the majority of isolates being 128 µg/mL. spp. showed a relatively low rate of resistance to nalidixic acid (48/86; 55.8%) when compared with isolates (179/205; 87.3%). Out of 145 isolates tested, we found in 11 (7.58%), in 8 (5.51%), in 4 (2.75%), in one (0.6%) and in none of the isolates. Of these 3 isolates were carbapenem sensitive, the rest were resistant.

CONCLUSION

Most of the isolates were sensitive to fosfomycin, carbapenems and resistant to cephalosporins and nalidixic acid. We detected carbapenemase genes in 13 (59%) out of 22 carbapenem resistant isolates and 3 (2.4%) out of 123 carbapenem sensitive isolates.