School of Biotechnology and Bioinformatics, D. Y. Patil Deemed to be University, Plot No. 50, Sector 15, CBD Belapur, 400614 Navi Mumbai, Maharashtra, India.
Amity Institute of Biotechnology, Amity University Maharashtra, Mumbai Pune Expressway, Bhatan, Post Somatane, Panvel, Mumbai, Maharashtra 410206, India.
Biomed Res Int. 2022 Jul 25;2022:1958939. doi: 10.1155/2022/1958939. eCollection 2022.
An anthraquinone textile dye, Reactive Blue 4 (RB4), poses environmental health hazards. In this study, remediation of RB4 (30-110 ppm) was carried out by hairy roots (HRs). UV-visible spectroscopy and FTIR analysis showed that the dye undergoes decolourization followed by degradation. In addition, toxicity and safety analyses of the bioremediated dye were performed on and zebrafish embryos, which revealed lesser toxicity of the bioremediated dye as compared to untreated dye. For , the highest concentration, i.e., 110 ppm of the treated dye, showed less chromosomal aberrations with a mitotic index of 8.5 ± 0.5, closer to control. Two-fold decrease in mortality of zebrafish embryos was observed at the highest treated dye concentration indicating toxicity mitigation. A higher level of lipid peroxidation (LPO) was recorded in the zebrafish embryo when exposed to untreated dye, suggesting a possible role of oxidative stress-inducing mortality of embryos. Further, the level of LPO was significantly normalized along with the other antioxidant enzymes in embryos after dye bioremediation. At lower concentrations, mitigated samples displayed similar antioxidant activity comparable to control underlining the fact that the dye at lesser concentration can be more easily degraded than the dye at higher concentration.
一种蒽醌类纺织染料，活性蓝 4（RB4），对环境健康构成危害。本研究采用发根（HR）对 RB4（30-110ppm）进行修复。紫外可见光谱和傅里叶变换红外分析表明，该染料发生了脱色和降解。此外，对生物修复染料进行了毒性和安全性分析，结果表明，与未处理染料相比，生物修复染料的毒性较小。对于蚕豆根尖细胞，最高浓度，即 110ppm 的处理染料，染色体畸变较少，有丝分裂指数为 8.5±0.5，更接近对照。在最高处理染料浓度下，斑马鱼胚胎的死亡率降低了两倍，表明毒性减轻。未处理染料处理的斑马鱼胚胎中脂质过氧化（LPO）水平较高，表明氧化应激可能导致胚胎死亡。此外，在染料生物修复后，胚胎中的 LPO 水平与其他抗氧化酶一起显著正常化。在较低浓度下，减轻的样本显示出与对照相似的抗氧化活性，这表明在较低浓度下染料更容易降解，而在较高浓度下染料则更难降解。
