Patil Pratibha, Desai Neetin, Govindwar Sanjay, Jadhav Jyoti Prafulla, Bapat Vishwas
Department of Microbiology, Shivaji University, Kolhapur 416004, India.
Planta. 2009 Sep;230(4):725-35. doi: 10.1007/s00425-009-0980-9. Epub 2009 Jul 18.
Tagetes patula L. (Marigold) hairy roots were selected among few hairy root cultures from other plants tested for the decolorization of Reactive Red 198. Hairy roots of Tagetes were able to remove dye concentrations up to 110 mg L(-l) and could be successively used at least for five consecutive decolorization cycles. The hairy roots of Tagetes decolorized six different dyes, viz. Golden Yellow HER, Methyl Orange, Orange M2RL, Navy Blue HE2R, Reactive Red M5B and Reactive Red 198. Significant induction of the activity of biotransformation enzymes indicated their crucial role in the dye metabolism. UV-vis spectroscopy, HPLC and FTIR spectroscopy analyses confirmed the degradation of Reactive Red 198. A possible pathway for the biodegradation of Reactive Red 198 has been proposed with the help of GC-MS and metabolites identified as 2-aminonaphthol, p-aminovinylsulfone ethyl disulfate and 1-aminotriazine, 3-pyridine sulfonic acid. The phytotoxicity study demonstrated the non-toxic nature of the extracted metabolites. The use of such hairy root cultures with a high ability for bioremediation of dyes is discussed.
在测试的几种其他植物的毛状根培养物中，万寿菊（孔雀草）的毛状根被选用于活性红198的脱色。万寿菊的毛状根能够去除高达110 mg L⁻¹的染料浓度，并且可以连续至少使用五个脱色循环。万寿菊的毛状根能使六种不同的染料脱色，即金黄HER、甲基橙、橙M2RL、海军蓝HE2R、活性红M5B和活性红198。生物转化酶活性的显著诱导表明它们在染料代谢中起关键作用。紫外可见光谱、高效液相色谱和傅里叶变换红外光谱分析证实了活性红198的降解。借助气相色谱-质谱联用仪提出了活性红198的一种可能的生物降解途径，鉴定出的代谢产物为2-氨基萘酚、对氨基乙烯砜二硫酸乙酯和1-氨基三嗪-3-吡啶磺酸。植物毒性研究表明所提取代谢产物无毒性。文中讨论了使用这种对染料具有高生物修复能力的毛状根培养物的情况。
