Yang Hui, Lin Xiu-Jiao, Liu Qiong, Yu Hao
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Research Center of Dental Esthetics and Biomechanics, Fujian Medical University, Fuzhou, China.
Clin Oral Investig. 2023 Mar;27(3):1005-1012. doi: 10.1007/s00784-022-04657-w. Epub 2022 Aug 4.
This in situ study aimed to evaluate the effects of the inhibitors of matrix metalloproteinases (MMPs) and cysteine cathepsins on dentin erosion.
Ten volunteers participated in this study. Each volunteer wore an intraoral appliance containing 4 dentin specimens subjected to different treatments: deionized water as a control, 1 mM 1,10-phenanthroline (an MMP inhibitor), 50 µM E-64 (a cysteine cathepsin inhibitor), and 1 mM 1,10-phenanthroline + 50 µM E-64. The specimens were dipped in 5 ml of the respective solutions for 30 min at room temperature and then exposed to in vivo erosive challenges by rinsing with 150 ml of a cola drink (4 × 5 min/day) for 7 days. The substance loss of the specimens was measured by profilometry. The transverse sections of the specimens were examined using scanning electron microscopy. Thereafter, the demineralized organic matrix (DOM) of the specimens was removed using type I collagen enzyme and assessed by performing profilometry. The differences in substance loss and DOM thickness among the groups were analyzed by one-way repeated-measures analysis of variance (ANOVA) and Bonferroni's test at a level of P < 0.05.
Protease inhibitors significantly reduced substance loss in comparison to that of the control group (all P < 0.05). A significantly thicker DOM was observed for the specimens treated with protease inhibitors than for the control specimens (all P < 0.05). No significant differences in substance loss or DOM thickness were found among the MMP inhibitor, cysteine cathepsin inhibitor, and MMP + cysteine cathepsin inhibitor groups.
The use of MMP and cysteine cathepsin inhibitors was shown to increase the acid resistance of human dentin, which may be due to the preservation of the DOM.
The application of protease inhibitors could be considered an appropriate preventive strategy for dentin erosion.
本原位研究旨在评估基质金属蛋白酶（MMPs）抑制剂和半胱氨酸组织蛋白酶抑制剂对牙本质侵蚀的影响。
10名志愿者参与了本研究。每名志愿者佩戴一个口腔内装置，其中包含4个经过不同处理的牙本质标本：去离子水作为对照，1 mM 1,10 - 菲咯啉（一种MMP抑制剂），50 μM E - 64（一种半胱氨酸组织蛋白酶抑制剂），以及1 mM 1,10 - 菲咯啉 + 50 μM E - 64。将标本在室温下于5 ml各自的溶液中浸泡30分钟，然后通过用150 ml可乐饮料冲洗（每天4次，每次5分钟）进行7天的体内侵蚀挑战。通过轮廓测定法测量标本的物质损失。使用扫描电子显微镜检查标本的横断面。此后，使用I型胶原酶去除标本的脱矿有机基质（DOM），并通过轮廓测定法进行评估。通过单向重复测量方差分析（ANOVA）和Bonferroni检验分析各组之间物质损失和DOM厚度的差异，P值 < 0.05。
与对照组相比，蛋白酶抑制剂显著减少了物质损失（所有P < 0.05）。观察到用蛋白酶抑制剂处理的标本的DOM比对照标本显著更厚（所有P < 0.05）。在MMP抑制剂、半胱氨酸组织蛋白酶抑制剂和MMP + 半胱氨酸组织蛋白酶抑制剂组之间，物质损失或DOM厚度没有发现显著差异。
使用MMP和半胱氨酸组织蛋白酶抑制剂可增加人牙本质的耐酸性，这可能是由于DOM的保留。
蛋白酶抑制剂的应用可被认为是一种合适的牙本质侵蚀预防策略。
