• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高胆固醇血症小鼠动脉粥样硬化量化的综述

A mini-review on quantification of atherosclerosis in hypercholesterolemic mice.

作者信息

Chen Hui, Howatt Deborah A, Franklin Michael K, Amioka Naofumi, Sawada Hisashi, Daugherty Alan, Lu Hong S

机构信息

Saha Cardiovascular Research Center, University of Kentucky, Lexington, Kentucky, United States.

Saha Aortic Center, University of Kentucky, Lexington, Kentucky, United States.

出版信息

Glob Transl Med. 2022;1(1). doi: 10.36922/gtm.v1i1.76. Epub 2022 Jun 14.

DOI:10.36922/gtm.v1i1.76
PMID:35925518
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9345319/
Abstract

Atherosclerosis is a leading cause of morbidity and mortality in many countries. Mice are the most frequently used animal model to study the pathogenesis and molecular mechanisms of atherosclerosis. analyses of the aorta and cross-sections of the aortic root are the two common modes for quantifying the severity of atherosclerosis in mice. This mini-review introduces these two methods, discusses their pros and cons, and provides suggestions to optimize the quantification of atherosclerosis, thereby enhancing rigor and reproducibility in preclinical research.

摘要

动脉粥样硬化是许多国家发病和死亡的主要原因。小鼠是研究动脉粥样硬化发病机制和分子机制最常用的动物模型。对主动脉和主动脉根部横截面的分析是量化小鼠动脉粥样硬化严重程度的两种常见方式。本综述介绍这两种方法，讨论其优缺点，并提供优化动脉粥样硬化量化的建议，从而提高临床前研究的严谨性和可重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21b/9345319/40ea53a2f124/nihms-1816986-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21b/9345319/70d9488442d9/nihms-1816986-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21b/9345319/40ea53a2f124/nihms-1816986-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21b/9345319/70d9488442d9/nihms-1816986-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21b/9345319/40ea53a2f124/nihms-1816986-f0002.jpg

相似文献

1
A mini-review on quantification of atherosclerosis in hypercholesterolemic mice.高胆固醇血症小鼠动脉粥样硬化量化的综述
Glob Transl Med. 2022;1(1). doi: 10.36922/gtm.v1i1.76. Epub 2022 Jun 14.
2
A volumetric method for quantifying atherosclerosis in mice by using microCT: comparison to en face.一种利用 microCT 对小鼠动脉粥样硬化进行定量分析的体视学方法：与直接检测法的比较。
PLoS One. 2011 Apr 18;6(4):e18800. doi: 10.1371/journal.pone.0018800.
3
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root.油红O及苏木精-伊红染色用于定量小鼠主动脉和主动脉根部的动脉粥样硬化负担
Methods Mol Biol. 2015;1339:85-99. doi: 10.1007/978-1-4939-2929-0_5.
4
Practical assessment of the quantification of atherosclerotic lesions in apoE⁻/⁻ mice.载脂蛋白E基因敲除小鼠动脉粥样硬化病变定量的实际评估
Mol Med Rep. 2015 Oct;12(4):5298-306. doi: 10.3892/mmr.2015.4084. Epub 2015 Jul 16.
5
Induction of atherosclerosis by low-fat, semisynthetic diets in LDL receptor-deficient C57BL/6J and FVB/NJ mice: comparison of lesions of the aortic root, brachiocephalic artery, and whole aorta (en face measurement).低脂半合成饮食诱导低密度脂蛋白受体缺陷的C57BL/6J和FVB/NJ小鼠动脉粥样硬化：主动脉根部、头臂动脉和整个主动脉病变的比较（正面测量）
Arterioscler Thromb Vasc Biol. 2003 Oct 1;23(10):1907-13. doi: 10.1161/01.ATV.0000090126.34881.B1. Epub 2003 Aug 7.
6
Combined effects of cholesterol reduction and apolipoprotein A-I expression on atherosclerosis in LDL receptor deficient mice.胆固醇降低和载脂蛋白A-I表达对低密度脂蛋白受体缺陷小鼠动脉粥样硬化的联合作用。
Atherosclerosis. 2002 Nov;165(1):15-22. doi: 10.1016/s0021-9150(02)00103-x.
7
Quantitative assay for mouse atherosclerosis in the aortic root.主动脉根部小鼠动脉粥样硬化的定量测定。
Methods Mol Med. 2006;129:83-95. doi: 10.1385/1-59745-213-0:83.
8
Uremia modulates the phenotype of aortic smooth muscle cells.尿毒症调节主动脉平滑肌细胞的表型。
Atherosclerosis. 2017 Feb;257:64-70. doi: 10.1016/j.atherosclerosis.2016.12.022. Epub 2016 Dec 23.
9
Anti-atherosclerotic effects of pravastatin in brachiocephalic artery in comparison with en face aorta and aortic roots in ApoE/LDLR mice.普伐他汀对载脂蛋白E/低密度脂蛋白受体双敲除小鼠头臂动脉的抗动脉粥样硬化作用：与主动脉弓及主动脉根部的比较
Pharmacol Rep. 2017 Feb;69(1):112-118. doi: 10.1016/j.pharep.2016.09.014. Epub 2016 Sep 17.
10
Molecular imaging of inflammation in atherosclerosis with targeted ultrasound detection of vascular cell adhesion molecule-1.通过靶向超声检测血管细胞黏附分子-1对动脉粥样硬化炎症进行分子成像
Circulation. 2007 Jul 17;116(3):276-84. doi: 10.1161/CIRCULATIONAHA.106.684738. Epub 2007 Jun 25.

引用本文的文献

1
Artery segmentation and atherosclerotic plaque quantification using AI for murine whole slide images stained with oil red O.使用人工智能对油红O染色的小鼠全切片图像进行动脉分割和动脉粥样硬化斑块定量分析。
Sci Rep. 2025 Apr 23;15(1):14152. doi: 10.1038/s41598-025-93967-6.
2
Risk factors for cerebral artery stenosis in patients with minor ischemic stroke classified as large atherosclerosis.归类为大动脉粥样硬化的轻度缺血性卒中患者脑动脉狭窄的危险因素。
Postepy Kardiol Interwencyjnej. 2025 Mar;21(1):94-100. doi: 10.5114/aic.2024.147643. Epub 2025 Feb 28.
3
Renal Proximal Tubule Cell-Specific Megalin Deletion Does Not Affect Atherosclerosis But Induces Tubulointerstitial Nephritis in Mice Fed a Western Diet.

本文引用的文献

1
Updates on Approaches for Studying Atherosclerosis.动脉粥样硬化研究方法的最新进展。
Arterioscler Thromb Vasc Biol. 2019 Apr;39(4):e108-e117. doi: 10.1161/ATVBAHA.119.312001.
2
Recommendation on Design, Execution, and Reporting of Animal Atherosclerosis Studies: A Scientific Statement From the American Heart Association.《动物动脉粥样硬化研究的设计、实施及报告建议：美国心脏协会科学声明》
Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):e131-e157. doi: 10.1161/ATV.0000000000000062. Epub 2017 Jul 20.
3
Hypercholesterolemia Induced by a PCSK9 Gain-of-Function Mutation Augments Angiotensin II-Induced Abdominal Aortic Aneurysms in C57BL/6 Mice-Brief Report.
肾近端小管细胞特异性巨蛋白缺失不影响动脉粥样硬化，但在喂食西方饮食的小鼠中诱发肾小管间质性肾炎。
Arterioscler Thromb Vasc Biol. 2025 Jan;45(1):74-89. doi: 10.1161/ATVBAHA.124.321366. Epub 2024 Nov 21.
4
Renal Proximal Tubule Cell-specific Megalin Deletion Does Not Affect Atherosclerosis But Induces Tubulointerstitial Nephritis in Mice Fed Western Diet.肾近端小管细胞特异性巨蛋白缺失不影响动脉粥样硬化，但在喂食西式饮食的小鼠中诱发肾小管间质性肾炎。
bioRxiv. 2024 Sep 27:2024.05.11.592234. doi: 10.1101/2024.05.11.592234.
5
Trehalose promotes atherosclerosis regression in female mice.海藻糖可促进雌性小鼠的动脉粥样硬化消退。
Front Cardiovasc Med. 2024 Feb 16;11:1298014. doi: 10.3389/fcvm.2024.1298014. eCollection 2024.
6
Endothelial nitric oxide synthase (eNOS) S1176 phosphorylation status governs atherosclerotic lesion formation.内皮型一氧化氮合酶（eNOS）S1176位点的磷酸化状态决定动脉粥样硬化病变的形成。
Front Cardiovasc Med. 2023 Nov 14;10:1279868. doi: 10.3389/fcvm.2023.1279868. eCollection 2023.
7
Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic mice.对肾近端小管中肾素血管紧张素系统各组分进行调控，无法改变高胆固醇血症小鼠的动脉粥样硬化情况。
Front Cardiovasc Med. 2023 Aug 16;10:1250234. doi: 10.3389/fcvm.2023.1250234. eCollection 2023.
8
Functional Exploration of Conserved Sequences in the Distal Face of Angiotensinogen-Brief Report.血管紧张素原远端保守序列的功能探索——简短报告。
Arterioscler Thromb Vasc Biol. 2023 Aug;43(8):1524-1532. doi: 10.1161/ATVBAHA.122.318930. Epub 2023 Jun 22.
9
Antisense oligonucleotides targeting hepatic angiotensinogen reduce atherosclerosis and liver steatosis in hypercholesterolemic mice.靶向肝脏血管紧张素原的反义寡核苷酸可减轻高胆固醇血症小鼠的动脉粥样硬化和肝脏脂肪变性。
Glob Transl Med. 2023;2(1). doi: 10.36922/gtm.288. Epub 2023 Feb 24.
PCSK9功能获得性突变诱导的高胆固醇血症加剧C57BL/6小鼠中血管紧张素II诱导的腹主动脉瘤——简要报告
Arterioscler Thromb Vasc Biol. 2016 Sep;36(9):1753-7. doi: 10.1161/ATVBAHA.116.307613. Epub 2016 Jul 28.
4
A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification.单次注射功能获得性突变型前蛋白转化酶枯草溶菌素9腺相关病毒载体可在未进行基因改造的小鼠中诱导心血管钙化。
Atherosclerosis. 2016 Aug;251:109-118. doi: 10.1016/j.atherosclerosis.2016.06.011. Epub 2016 Jun 9.
5
Atherosclerosis.动脉粥样硬化
Arterioscler Thromb Vasc Biol. 2015 Mar;35(3):485-91. doi: 10.1161/ATVBAHA.115.305380.
6
Accelerating the pace of atherosclerosis research.加快动脉粥样硬化研究的步伐。
Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):11-2. doi: 10.1161/ATVBAHA.114.304833.
7
Induction of sustained hypercholesterolemia by single adeno-associated virus-mediated gene transfer of mutant hPCSK9.通过单腺相关病毒介导的突变型人前蛋白转化酶枯草溶菌素9基因转移诱导持续性高胆固醇血症
Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):50-9. doi: 10.1161/ATVBAHA.114.303617. Epub 2014 Oct 23.
8
AAV vectors expressing LDLR gain-of-function variants demonstrate increased efficacy in mouse models of familial hypercholesterolemia.AAV 载体表达 LDLR 获得性功能变异体在家族性高胆固醇血症的小鼠模型中显示出更高的疗效。
Circ Res. 2014 Aug 29;115(6):591-9. doi: 10.1161/CIRCRESAHA.115.304008. Epub 2014 Jul 14.
9
Induction of atherosclerosis in mice and hamsters without germline genetic engineering.在不进行种系基因工程的情况下诱导小鼠和仓鼠发生动脉粥样硬化。
Circ Res. 2014 May 23;114(11):1684-9. doi: 10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27.
10
Stabilisation of atherosclerotic plaques. Position paper of the European Society of Cardiology (ESC) Working Group on atherosclerosis and vascular biology.动脉粥样硬化斑块的稳定。欧洲心脏病学会（ESC）动脉粥样硬化和血管生物学工作组立场文件。
Thromb Haemost. 2011 Jul;106(1):1-19. doi: 10.1160/TH10-12-0784. Epub 2011 Jun 14.