MOE Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
J Phys Chem Lett. 2022 Aug 18;13(32):7386-7391. doi: 10.1021/acs.jpclett.2c01873. Epub 2022 Aug 4.
Ionic liquids (ILs), although being a class of promising green solvents, have received many reports on the toxicity to living organisms. In this work, aiming at elucidating the disruptive effect of ILs to cell membrane lipid rafts, we investigated the effect of three 1-octylimidazolium-based ILs on the properties of the liquid ordered phase (L, a commonly used lipid raft model) of egg sphingomyelin (SM)-cholesterol model membrane. We found that, in the absence of cholesterol, a very low IL:SM molar ratio of 0.01:1 could disrupt the integrity of the bilayer structure. In sharp contrast, the presence of cholesterol in lipid bilayers helps the L phase resist the damaging effect of the ILs. For the role of the IL headgroup, we found that the mono- and trisubstituted species show a stronger destructive effect on the structures of the model rafts than the commonly used disubstituted counterpart.
离子液体（ILs）虽然是一类有前途的绿色溶剂，但已有许多关于其对生物体毒性的报道。在这项工作中，我们旨在阐明 ILs 对细胞膜脂筏的破坏作用，研究了三种基于 1-辛基咪唑的 ILs 对卵鞘磷脂（SM）-胆固醇模型膜中液相有序相（L，一种常用的脂筏模型）性质的影响。我们发现，在没有胆固醇的情况下，非常低的 IL:SM 摩尔比 0.01:1 就可以破坏双层结构的完整性。相比之下，胆固醇在脂质双层中的存在有助于 L 相抵抗 IL 对其的破坏作用。对于 IL 头基的作用，我们发现单取代和三取代的物种比常用的双取代的物种对模型筏结构具有更强的破坏作用。
