Department of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, Germany.
Septomics Research Centre, Jena University Hospital, Jena, Germany.
BMJ Open. 2022 Jun 3;12(6):e062592. doi: 10.1136/bmjopen-2022-062592.
Obesity, defined as a body mass index ≥30 kg/m, is one of the most prevalent health conditions worldwide. It is part of the metabolic syndrome, which encompasses arterial hypertension, dyslipoproteinaemia and diabetes. Obesity is viewed as a systemic disease with pathophysiological mechanisms on the molecular level. Dysfunction of the mitochondrion and systemic low-grade inflammation are among the proposed causes for the metabolic changes. In severe cases of obesity, laparoscopic sleeve gastrectomy, a bariatric operation, can achieve the desired weight loss and has been associated with clinical outcome improvement. Hitherto, the influence of patients' body composition on mitochondrial function and concomitant metabolic changes has not been fully understood. This study aims to quantify the patient's body composition before and after laparoscopic sleeve gastrectomy and to correlate these findings with changes in mitochondrial oxygen metabolism, metabolome and immune status.
In this prospective monocentric cohort study, patients undergoing laparoscopic sleeve gastrectomy (n=30) at Jena University Hospital (Germany) will be assessed before surgery and at four time points during a 1-year follow-up. Body composition will be measured by bioimpedance analysis. Non-invasive assessment of mitochondrial oxygen metabolism using protoporphyrin IX-triplet state lifetime technique (PPIX-TSLT) and blood sampling for, among other, metabolomic and immunological analysis, will be performed. The primary outcome is the difference in relative fat mass between the preoperative time point and 6 months postoperatively. Further outcomes comprise longitudinal changes of PPIX-TSLT and metabolic and immunological variables. Outcomes will be assessed using paired t-tests, Wilcoxon signed-rank tests and regression analyses.
The study was approved by the Ethics Committee of Friedrich Schiller University Jena (2018-1192-BO). Written informed consent will be obtained from all patients prior to enrolment in the study. The results will be published in peer-reviewed journals and presented at appropriate conferences.
DRKS00015891.
肥胖定义为身体质量指数（BMI）≥30kg/m2，是全球最普遍的健康问题之一。它是代谢综合征的一部分，包括动脉高血压、血脂异常和糖尿病。肥胖被视为一种系统性疾病，其分子水平的病理生理机制。线粒体功能障碍和全身低度炎症被认为是代谢变化的原因之一。在严重肥胖的情况下，腹腔镜袖状胃切除术，一种减肥手术，可以达到预期的体重减轻，并与临床结果改善相关。迄今为止，患者的身体成分对线粒体功能和伴随的代谢变化的影响尚未完全理解。本研究旨在定量测量腹腔镜袖状胃切除术前和术后患者的身体成分，并将这些发现与线粒体氧代谢、代谢组和免疫状态的变化相关联。
在这项前瞻性单中心队列研究中，德国耶拿大学医院（Jena University Hospital）接受腹腔镜袖状胃切除术的患者（n=30）将在手术前和 1 年随访期间的 4 个时间点进行评估。身体成分将通过生物阻抗分析进行测量。使用原卟啉 IX-三重态寿命技术（PPIX-TSLT）进行非侵入性评估线粒体氧代谢，并进行血液采样，用于代谢组学和免疫学分析等。主要结果是术前时间点与术后 6 个月之间相对脂肪量的差异。进一步的结果包括 PPIX-TSLT 和代谢及免疫变量的纵向变化。结果将使用配对 t 检验、Wilcoxon 符号秩检验和回归分析进行评估。
该研究得到了耶拿弗里德里希·席勒大学（Friedrich Schiller University Jena）伦理委员会的批准（2018-1192-BO）。所有患者在入组研究前将获得书面知情同意。研究结果将发表在同行评议的期刊上，并在适当的会议上展示。
DRKS00015891。
