Li Xiaofeng, Shi Xiaoli, Mesalam Noura M, Liu Lei, Chen Zhihao, Yang Bing
College of Animal Science, Anhui Science and Technology University, Fengyang, China.
College of Animal Science, Guizhou University, Guiyang, China.
Front Vet Sci. 2022 Jul 19;9:946148. doi: 10.3389/fvets.2022.946148. eCollection 2022.
Lysoforte (LFT) plays a vital role in maintaining broilers' health and intestinal morphology. However, the mechanism behind the effects of LFT improving intestinal morphology and health is still unclear. Therefore, this study was implemented to explore the central genes linked to the regulatory effect of LFT. Seventy-five newly hatched Cobb 500 male broilers were randomly divided into three groups: control, LFT500, and LFT1000 groups, with 25 chicks per group. The control chicks were provided with the basal diet, and the birds in LFT500 and LFT1000 groups were offered the same basal diet with 500 g/ton and 1,000 g/ton LFT, respectively. GSE94622 dataset consisted of the control and two LFT-treated groups (LFT500 and LFT1000). Jejuna samples were obtained from Gene Expression Omnibus (GEO). Totally 106-344 DEGs were obtained by comparing LFT500 and LFT1000 vs. control and LFT1000 vs. LFT500. Gene ontology (GO) enrichment suggested that the DEGs are mainly related to the phosphatidylethanolamine biosynthetic process and neuron projection extension. KEGG analysis suggested the DEGs were enriched in AGE-RAGE, fatty acid elongation, ECM-receptor interaction (ECMRI), glycerophospholipid metabolism, focal adhesion, unsaturated fatty acids biosynthesis, and ABC transporters. Moreover, 29 genes, such as , and , might be closely related to promoting jejuna morphology in broilers. Taken together, our observation enhances the understanding of LFT in maintaining intestinal architecture and the general health of broiler chickens.
溶菌福泰（LFT）在维持肉鸡健康和肠道形态方面发挥着至关重要的作用。然而，LFT改善肠道形态和健康的作用机制仍不清楚。因此，本研究旨在探索与LFT调节作用相关的核心基因。75只刚孵化的科宝500雄性肉鸡被随机分为三组：对照组、LFT500组和LFT1000组，每组25只雏鸡。对照组雏鸡饲喂基础日粮，LFT500组和LFT1000组的鸡分别饲喂添加了500克/吨和1000克/吨LFT的相同基础日粮。GSE94622数据集由对照组和两个LFT处理组（LFT500和LFT1000）组成。空肠样本取自基因表达综合数据库（GEO）。通过比较LFT500和LFT1000组与对照组以及LFT1000组与LFT500组，共获得106 - 344个差异表达基因（DEG）。基因本体（GO）富集分析表明，这些DEG主要与磷脂酰乙醇胺生物合成过程和神经元投射延伸有关。京都基因与基因组百科全书（KEGG）分析表明，这些DEG富集于晚期糖基化终末产物受体（AGE-RAGE）、脂肪酸延长、细胞外基质-受体相互作用（ECMRI）、甘油磷脂代谢、粘着斑、不饱和脂肪酸生物合成和ABC转运蛋白等通路。此外，29个基因，如……，可能与促进肉鸡空肠形态密切相关。综上所述，我们的观察结果加深了对LFT在维持肉鸡肠道结构和整体健康方面的理解。
