Wardhani Sabrina Wahyu, Wongsakul Boonyakorn, Kasantikul Tanit, Piewbang Chutchai, Techangamsuwan Somporn
The International Graduate Program of Veterinary Science and Technology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Animal Virome and Diagnostic Development Research Group, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Front Vet Sci. 2021 Aug 19;8:710701. doi: 10.3389/fvets.2021.710701. eCollection 2021.
Throughout the year, the Thai Red Cross Society (TRCS), Bangkok, Thailand, received more than 100 animals that died of suspected rabies due to neurological clinical signs. Concerning the role of viral infection in the brain in the outcome of neurological diseases in cats and dogs, a comprehensive study was conducted of 107 brain samples of cats and dogs submitted to the TRCS from August 2019 to August 2020. Selective molecular screening using conventional polymerase chain reaction (PCR) and reverse transcription PCR targeting nine viral pathogens was employed in addition to histopathological investigations. The results showed that (CPPV-1) was detected in 18.69% of the cats and dogs sampled (20/107). These results were found in young and old animals; the brain tissue did not show any pathological changes suggesting encephalitis or cerebellar hypoplasia. In addition, , and were also detected, providing a broader range of potential viral infections to consider in the clinical manifestation of neurological disorders in companion animals. The detection of all pathogens was confirmed by the localization of each viral antigen in various resident brain cells using immunohistochemistry. A unique L582S amino acid substitution of the non-structural protein 1 gene coding sequence, speculated to be associated with the neurotropism of CPPV-1 in cats and dogs, was not evident. In conclusion, this study revealed a noteworthy neurotropism of CPPV-1 in both cats and dogs without neurological lesions.
全年，泰国曼谷的泰国红十字会（TRCS）接收了100多只因神经临床症状疑似死于狂犬病的动物。关于脑部病毒感染在猫和狗神经疾病结局中的作用，对2019年8月至2020年8月提交给TRCS的107份猫和狗的脑样本进行了一项全面研究。除了组织病理学调查外，还采用了针对9种病毒病原体的常规聚合酶链反应（PCR）和逆转录PCR进行选择性分子筛查。结果显示，在采样的猫和狗中，18.69%（20/107）检测到犬细小病毒1型（CPPV-1）。这些结果在年轻和老年动物中均有发现；脑组织未显示任何提示脑炎或小脑发育不全的病理变化。此外，还检测到了其他病毒，这为伴侣动物神经疾病临床表现中需要考虑的潜在病毒感染范围提供了更广泛的信息。通过免疫组织化学在各种驻留脑细胞中定位每种病毒抗原，证实了所有病原体的检测。推测与CPPV-1在猫和狗中的嗜神经性相关的非结构蛋白1基因编码序列的独特L582S氨基酸取代并不明显。总之，本研究揭示了CPPV-1在猫和狗中具有值得注意的嗜神经性，且无神经病变。
