Wei Fuxiao, Yang Xinyue, Zhang Meihong, Xu Chang, Hu Yongfei, Liu Dan
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Microbiol. 2022 Jul 19;13:927245. doi: 10.3389/fmicb.2022.927245. eCollection 2022.
() has shown potential as a probiotic for the prevention and treatment of non-alcoholic fatty liver disease in both humans and mice. However, relatively little is known about the effects of on lipid metabolism, productivity, and product quality in laying hens. In this study, we explored whether supplementation could improve lipid metabolism and egg quality in laying hens and sought to identify the underlying mechanism. In the first experiment, 80 Hy-Line Brown laying hens were divided into four groups, one of which was fed a normal diet (control group), while the other three groups were administered a high-energy, low-protein diet to induce fatty liver hemorrhagic syndrome (FLHS). Among the three FLHS groups, one was treated with phosphate-buffered saline, one with live , and one with pasteurized . In the second experiment, 140 Hy-Line Brown laying hens were divided into two groups and respectively fed a basal diet supplemented or not with lyophilized powder. The results showed that, in laying hens with FLHS, treatment with either live or pasteurized efficiently decreased body weight, abdominal fat deposition, and lipid content in both serum and the liver; downregulated the mRNA expression of lipid synthesis-related genes and upregulated that of lipid transport-related genes in the liver; promoted the growth of short-chain fatty acids (SCFAs)-producing microorganisms and increased the cecal SCFAs content; and improved the yolk lipid profile. Additionally, the supplementation of lyophilized powder of to aged laying hens reduced abdominal fat deposition and total cholesterol (TC) levels in both serum and the liver, suppressed the mRNA expression of cholesterol synthesis-related genes in the liver, reduced TC content in the yolk, increased eggshell thickness, and reshaped the composition of the gut microbiota. Collectively, our findings demonstrated that can modulate lipid metabolism, thereby, promoting laying hen health as well as egg quality and nutritive value. Live, pasteurized, and lyophilized preparations all have the potential for use as additives for improving laying hen production.
（）已显示出作为益生菌在预防和治疗人类及小鼠非酒精性脂肪性肝病方面的潜力。然而，关于（）对蛋鸡脂质代谢、生产性能和产品质量的影响，人们了解相对较少。在本研究中，我们探讨了补充（）是否能改善蛋鸡的脂质代谢和蛋品质，并试图确定其潜在机制。在第一个实验中，80只海兰褐蛋鸡分为四组，其中一组饲喂正常日粮（对照组），而其他三组给予高能低蛋白日粮以诱导脂肪肝出血综合征（FLHS）。在三个FLHS组中，一组用磷酸盐缓冲盐水处理，一组用活的（）处理，一组用巴氏杀菌的（）处理。在第二个实验中，140只海兰褐蛋鸡分为两组，分别饲喂添加或不添加（）冻干粉的基础日粮。结果表明，在患有FLHS的蛋鸡中，用活的或巴氏杀菌的（）处理均能有效降低体重、腹部脂肪沉积以及血清和肝脏中的脂质含量；下调肝脏中脂质合成相关基因的mRNA表达并上调脂质转运相关基因的表达；促进产生短链脂肪酸（SCFA）的微生物生长并增加盲肠SCFA含量；并改善蛋黄脂质谱。此外，向老龄蛋鸡补充（）冻干粉可减少腹部脂肪沉积以及血清和肝脏中的总胆固醇（TC）水平，抑制肝脏中胆固醇合成相关基因的mRNA表达，降低蛋黄中的TC含量，增加蛋壳厚度，并重塑肠道微生物群的组成。总体而言，我们的研究结果表明（）可以调节脂质代谢，从而促进蛋鸡健康以及蛋品质和营养价值。活的、巴氏杀菌的和冻干的（）制剂都有作为改善蛋鸡生产添加剂的潜力。
