Lei Jilin, Fan Qianyi, Yu Jingyao, Ma Yan, Yin Junhui, Liu Rui
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China.
Front Microbiol. 2022 Jul 19;13:962146. doi: 10.3389/fmicb.2022.962146. eCollection 2022.
Nitrification inhibitor (NI) is often claimed to be efficient in mitigating nitrogen (N) losses from agricultural production systems by slowing down nitrification. Increasing evidence suggests that ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) have the genetic potential to produce nitrous oxide (NO) and perform the first step of nitrification, but their contribution to NO and nitrification remains unclear. Furthermore, both AOA and AOB are probably targets for NIs, but a quantitative synthesis is lacking to identify the "indicator microbe" as the best predictor of NI efficiency under different environmental conditions. In this present study, a meta-analysis to assess the response characteristics of AOB and AOA to NI application was conducted and the relationship between NI efficiency and the AOA and AOB A genes response under different conditions was evaluated. The dataset consisted of 48 papers (214 observations). This study showed that NIs on average reduced 58.1% of NO emissions and increased 71.4% of soil concentrations, respectively. When 3, 4-dimethylpyrazole phosphate (DMPP) was applied with both organic and inorganic fertilizers in alkaline medium soils, it had higher efficacy of decreasing NO emissions than in acidic soils. The abundance of AOB A genes was dramatically reduced by about 50% with NI application in most soil types. Decrease in NO emissions with NI addition was significantly correlated with AOB changes ( = 0.135, = 110, < 0.01) rather than changes in AOA, and there was an obvious correlation between the changes in concentration and AOB A gene abundance after NI application ( = 0.037, = 136, = 0.014). The results indicated the principal role of AOB in nitrification, furthermore, AOB would be the best predictor of NI efficiency.
硝化抑制剂（NI）通常被认为可以通过减缓硝化作用来有效减少农业生产系统中的氮（N）损失。越来越多的证据表明，氨氧化古菌（AOA）和氨氧化细菌（AOB）具有产生一氧化二氮（N₂O）和进行硝化作用第一步的遗传潜力，但其对N₂O和硝化作用的贡献仍不清楚。此外，AOA和AOB可能都是NI的作用靶点，但缺乏定量综合分析来确定“指示微生物”作为不同环境条件下NI效率的最佳预测指标。在本研究中，进行了一项荟萃分析以评估AOB和AOA对NI施用的响应特征，并评估了不同条件下NI效率与AOA和AOB A基因响应之间的关系。数据集由48篇论文（214个观测值）组成。本研究表明，NI平均分别减少了58.1%的N₂O排放和增加了71.4%的土壤 浓度。当在碱性介质土壤中同时施用有机肥料和无机肥料时，3,4-二甲基吡唑磷酸盐（DMPP）降低N₂O排放的效果比在酸性土壤中更高。在大多数土壤类型中，施用NI后AOB A基因的丰度显著降低了约50%。添加NI后N₂O排放的减少与AOB的变化显著相关（ = 0.135， = 110， < 0.01），而不是与AOA的变化相关，并且施用NI后 浓度的变化与AOB A基因丰度之间存在明显的相关性（ = 0.037， = 136， = 0.014）。结果表明AOB在硝化作用中起主要作用，此外，AOB将是NI效率的最佳预测指标。
