Makde Manjiri Milind, Sathawane Prajakta
Department of Pathology, Government Medical College, Nagpur, India.
Department of Pathology, Deenanath Mangeshkar Hospital and Research Centre, Pune, Maharashtra, India.
Cytojournal. 2022 Jun 14;19:41. doi: 10.25259/CMAS_03_16_2021. eCollection 2022.
Liquid-based cytology (LBC) is a monolayer slide preparation technology that has outperformed conventional Pap smears because of improved fixation, decreased obscuring factors, and standardized cell transfer. In LBC, samples are collected by completely immersing the sampling device into the company vial containing preservative fluid, whereby the cells are preserved and fixed simultaneously unlike conventional smears where the sample is smeared onto the glass slide and fixed separately. To date, two major liquid-based preparation methods are known - ThinPrep and SurePath. These two methods are different in their principles of cell harvesting but produce similar preparations. SurePath works on the principle of density gradient sedimentation. In this, a sample is vortexed and strained to break the mucus and large cell groups and then is treated through a density gradient centrifugation process to remove blood and debris. The cell pellet is resuspended and is allowed to sediment onto a glass slide. This is followed by staining on the PrepStain instrument. Government Medical College and Hospital, Nagpur, India, uses the SurePath method which was approved by FDA in the USA in 1999. Our institution uses Rovers Cervex-Brush to collect the cells from the transformation zone. This chapter describes the principle of SurePath and the processing of cervicovaginal specimen using the fully automated system in the laboratory.
液基细胞学（LBC）是一种单层玻片制备技术，由于其固定效果更好、干扰因素减少以及细胞转移标准化，其性能优于传统巴氏涂片。在LBC中，通过将采样装置完全浸入装有保存液的配套小瓶中来收集样本，这样细胞会同时被保存和固定，这与传统涂片不同，传统涂片是将样本涂抹在载玻片上然后单独固定。迄今为止，已知两种主要的液基制备方法——ThinPrep和SurePath。这两种方法在细胞收集原理上有所不同，但制备出的样本相似。SurePath基于密度梯度沉降原理工作。在此方法中，将样本涡旋并过滤以打破黏液和大细胞团，然后通过密度梯度离心过程处理以去除血液和碎片。细胞沉淀被重新悬浮，然后使其沉积在载玻片上。接下来在PrepStain仪器上进行染色。印度那格浦尔政府医学院及医院使用SurePath方法，该方法于1999年在美国获得FDA批准。我们机构使用Rovers Cervex-Brush从转化区收集细胞。本章描述SurePath的原理以及在实验室中使用全自动系统对宫颈阴道标本的处理过程。
