Department of Biochemistry and Molecular Biology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, Türkiye.
Department of Medical Biochemistry, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, Türkiye.
Int J Mol Sci. 2023 Jun 21;24(13):10473. doi: 10.3390/ijms241310473.
Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital's outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with 'negative for intraepithelial lesion or malignancy' (NILM), 21 samples with 'atypical squamous cells of undetermined significance' (ASC-US), and 33 samples with 'low-grade squamous intraepithelial lesion and worse' (≥LSIL) were analyzed, using cytology and the patients' histology reports. Highly accurate concordance was obtained for gold-standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.
宫颈癌检测的分诊方法显示出中等准确性，并且存在相当多的假阴性和假阳性结果率。补充诊断参数可以帮助提高识别需要治疗的患者的准确性。使用靶向蛋白质组学方法进行了一项初步研究，使用在医院门诊收集的机会性 ThinPrep 样本来确定微小染色体维持蛋白 3 (MCM3) 和 envoplakin (EVPL) 蛋白的浓度水平。对 40 份“无上皮内病变或恶性病变”(NILM)、21 份“非典型鳞状细胞意义不明”(ASC-US)和 33 份“低级别鳞状上皮内病变及更高级别病变”(≥LSIL)的样本进行了分析，分析方法为细胞学和患者的组织学报告。对于金标准确认样本，获得了高度准确的一致性，表明 MCM3/EVPL 比值可以区分非发育不良和发育不良的样本。因此，我们提出 MCM3 和 EVPL 是用于基于人群的宫颈癌筛查的有前途的候选蛋白生物标志物。
