Inayat Rehan, Khurshid Aroosa, Boamah Solomon, Zhang Shuwu, Xu Bingliang
College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, China.
Front Physiol. 2022 Jul 19;13:901115. doi: 10.3389/fphys.2022.901115. eCollection 2022.
Aphids are one of the most common insect pests in greenhouse and field crops worldwide, causing significant crop yields and economic losses. The objective of this study was to determine the mortality, enzymatic antioxidant activity and gene expression of cabbage aphids ( L.) in response to T6 (T6) at different time points from Day 1 to 7 after inoculation. Our results showed that the highest mortality of was observed on Day 7 at a concentration of 1 × 10 spores ml (73.31%) after inoculation with T6 compared with the control on Day 7 (11.51%). The activities of the enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and glutathione S-transferase (GST) were increased by 52.98%, 44.77%, 48.26%, 49.39%, 45.13% and 39.67%, respectively on Day 3 after inoculation with T6 compared to the control. Howerver increased days post treatment (dpt) decreased the activities of SOD POD CAT APX GPX and GST enzymes by 20.79%, 21.05%, 13.75%, 20.45%, 25.38%, and 19.76% repectively on Day 7 compared to control. The transcript levels of , , , , and genes were increased by 10.87, 9.87, 12.77, 6.22 and 4.07 respectively at Day 3 after inoculation with T6 in comparison to the control. However, the , , , , and transcription levels decreased by 0.43, 0.44, 0.35, 0.52 and 0.47 respectively, compared to control at Day 7 Our results suggest that the T6 strain has a potential effect on the antioxidant activity and mortality of and therefore could be used as a natural biocontrol agent against in the future.
蚜虫是全球温室和田间作物中最常见的害虫之一,会导致严重的作物减产和经济损失。本研究的目的是确定接种后第1天至第7天不同时间点,甘蓝蚜对T6的死亡率、酶促抗氧化活性和基因表达。我们的结果表明,接种T6后第7天,在浓度为1×10孢子/毫升时观察到最高死亡率(73.31%),而第7天的对照死亡率为11.51%。与对照相比,接种T6后第3天,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽S-转移酶(GST)的活性分别提高了52.98%、44.77%、48.26%、49.39%、45.13%和39.67%。然而,与对照相比,处理后天数增加(dpt),第7天SOD、POD、CAT、APX、GPX和GST酶的活性分别降低了20.79%、21.05%、13.75%、20.45%、25.38%和19.76%。与对照相比,接种T6后第3天,、、、和基因的转录水平分别提高了10.87、9.87、12.77、6.22和4.07。然而,与对照相比,第7天、、、和的转录水平分别下降了0.43、0.44、0.35、0.52和0.47。我们的结果表明,T6菌株对甘蓝蚜的抗氧化活性和死亡率有潜在影响,因此未来可作为防治甘蓝蚜的天然生物防治剂。